Characterization And Identification Of SRB And Its Control Method

Sulfate-reducing bacteria （SRB） is one of the harmful bacteria have caused materialcorrosion, the loss is increasing year by year, bactericide is the main method to controlSRB. The sensitivity of different kind of SRB to bactericide is different, long-time use afixed bactericide is easy to generate drug-resistant bacteria, in addition, the chemical itselfhas toxic, using it extensivily can cause secondary pollution. In order to control thecorrosion caused by SRB, the main content of this paper is studying Characterization andidentification of SRB, environment-friendly, efficient and broad-spectrum bactericide.The SRB is isolated from five oil fields of China, which is Characterizaed andidentified preliminarily according to its physiological and biochemical characteristics,combined with Fourier transform infrared spectroscopy （FT-IR）. The SRB is alsoidentified by Polymerase chain reaction （PCR） in molecular level, the primers based onthe six major groups16S rDNA gene sequence of SRB. The results show that the optimumgrowth temperature of Zhan Jiang SRB, Zhong Yuan SRB, Ji Lin SRB, Sheng Li SRB is37℃, the optimum pH is7.0; the optimum growth temperature of Bo Hai SRB is55℃,the optimum pH also is7.0.This paper synthesize two new quaternary phosphonium salt bactericides, which areTetrakis hydroxymethyl phosphonium sulfate （THPS） and Tributyl tetradecylphosphonium chloride （B350）, the antibacterial properties of bactericides and the toxicityof bactericide on green algae are studied. The results show that THPS has strongantibacterial properties to Desulfovibrio, the minimum inhibitary concentration （MIC） is60mg/L, Electrochemical impedance spectroscopy results show THPS has strong abilityto strip biofilms, but the antibacterial properties of THPS to Desulfotomaculum is notobvious. B350also has strong antibacterial properties to both Desulfovibrio andDesulfotomaculum, the MIC is10mg/L, it has strong antibacterial properties to SRBbiofilms too. the results show THPS to green algae:96h-EC₅₀=33.58mg/L and B350:96h-EC₅₀=6.88mg/L. According to the toxicity evaluation criteria of chemical to algae, THPS and B350is low toxicity in the practical application.