Study On Flavones Of Protentilla Freyniana Bornm

Protentilla freyniana Bornm is perennial herbs, located on the grasslands andmountain slopes of East China, central China and Southwest, with clearing anddetoxifying, and the folk put its rhizome or herb into medicine. But so far theProtentilla freyniana Bornm has not been accepted as a kind of Chinese herbalmedicine for the Chinese Pharmacopoeia (2000) or local standards, and the reports arerare at home and abroad about its chemical composition and biological activity. In thispaper, we study techniques of microwave extraction about flavones from Protentillafreyniana Bornm, the techniques of separation and purification of flavones fromProtentilla Freyniana Bornm, the component and the content of Protentilla freynianaBornm analysed by LC-MS, and the antioxidant activities and free radical scavengingof Protentilla Freyniana Bornm were also evaluated.The researches about flavones of Protentilla freyniana Bornm in this thesisincluded four aspects:(1) The microwave extraction of flavones from Protentilla freyniana Bornm wasstudied．The effect of the ethanol concentration, the microwave power, the extractiontime, the refluxing temperature and the ratio of material to liquid on microwaveextraction process of Protentilla freyniana Bornm was researched with single factorexperiment. The optimum conditions were:75%ethanol，material-liquid ratio1：25，70℃e xtracted for5minutes with microwave power400W.The average yield offlavones was4.24%．(2) To selected the best macroporous adsorption resin, we inspected the separation andpurification of6kinds of macroporous adsorption resin for the flavonoids ofProtentilla freyniana Bornm with the adsorption rate and desorption rate as an index,and detected the purification effect by HPLC analysis; Analyses of filtrationconcentration, pH, resin content and the concentration and pH of ethanol for theinfluence of adsorption and desorption have been studied. Experimental results showthat D101of macroporous adsorption resin has the best adsorption and desorptionperformance and the appropriate adsorption and desorption conditions is: adsorption liquid concentration to2.0mg/mL, pH=6, resin content and adsorption fluid volumeratio (g/mL) for1:30; Balance concentration to0.36mg/mL, namely resin has reachedadsorption saturation, saturated the adsorption quantity for49.1mg/g dry resins;ethanol concentration for50%, pH=6.(3) The kinds and contents of the flavones of Protentilla freyniana Bornm areidentified by high performance liquid chromatography-Mass Spectrometry (LC-MS).The results show that there are seven substances mainly, and they are Epicatechin,2,3,5,4-tetrahydroxyl diphenylethylene-2-o-glucoside, Vitexin, Hypericin, Rutin,Icariin and Astragaline, meanwhile their contents are73.02mg/g,14.53mg/g,15.05mg/g,12.51mg/g,160.35mg/g,27.10mg/g and144.52mg/g separately.(4) The antioxidant activities and free radical scavenging of Protentilla freynianaBornm and potentilla fulgens were evaluated based on total antioxidant ability, yolklipoprotein, hydroxyl radical and superoxide anion scav-enging activities, andscavenging DPPH radical. The various antioxidant activities were compared withsynthetic antioxidants such as ascorbic acid (Vc) and propyl gallate(PG).The resultsrevealed that within certain range of concentration, four kinds of Protentilla freynianaBornm compound in the first four aspects have a fixed order: Potentilla freyniana(Fujian)> Potentilla freyniana (Hebei)> potentilla fulgens (Hebei)> potentillafulgens (Fujian), but in the DPPH free radical scavenging a slightly different; andcompared to synthetic antioxidants such as Vc and PG, their activity performance issimilar.