| In this study, the Guangxi characteristic ripe Liubao tea was taken as raw materials. Firstly, the extraction, separation and mice experiments were made. Then separate, purify and determine the derived compounds to provide theory support and technical data for Liubao tea’s deep development, and to promote Liubao tea functional products’developing in the future.(1) Take Guangxi ripe Liubao tea as raw materials. Derive the extractum with70%ethanol, then adopt solvent separation system and use petroleum ether, ethyl acetate, n-butyl and distilled water to extract the extractum. At last, five different polarity parts would be obtained for the latter study. The extraction yield of ethanol leaching extracts was18.08%, petroleum ether extracts0.21%, ethyl acetate extracts0.64%, n-butyl extracts1.63%, and distilled water extracts14.51%. The result showed that the extracting method was simple and feasible, and the extracts of secondary polarity or higher would be more.(2) Build mice acute hyperlipoidemia model and feed the mice extracts with gavage. After14days, comparing model group with blank contrast group, it showed the model succeeded for the lipid levels increased obviously. Set0.3and0.15(g·kg-1·bw-1·d-1) as big and small dose respectively. Compare the tea extracts groups and the model group to figure out which solvent extracts can significantly lower the blood lipid level. As a result,0.3and0.15distilled water extracts,0.3ethanol leaching extracts and0.3ethyl acetate extracts all significantly reduced blood serum’s total cholesterol(TC) and triglyceride(TG), and increased high density lipoprotein cholesterol (HDL-C), while n-butanol and petroleum ether extracts just slightly reduced TC and TG, and HDL-C was not significantly influenced. The results showed that Liubao tea’s ethanol leaching extracts, ethyl acetate extracts and distilled water extracts all had significant blood lipid lowering activity.(3) Feed mice three Liubao tea blood lipid lowering extracts with gavage10days. Set0.4and0.2(g·kg-1·bw-1·d-1) as big and small dose respectively. Compare the tea extracts groups with the contrast groups to confirm whether these extracts can resist blood coagulation. As a result,0.4and0.2ethyl acetate extracts,0.4and0.2distilled water extracts, and0.4ethanol leaching extracts all significantly extended blood plasma’s activated partial thromboplastin time (APTT);0.4and0.2ethyl acetate extracts,0.4and0.2ethanol leaching extracts, and0.4distilled water extracts all significantly extended blood plasma’s thrombin time (TT);0.4and0.2ethyl acetate extracts,0.4ethanol leaching extracts and0.4distilled water extracts all significantly extended blood plasma’s pro-thrombin time (PT). The result showed that all the three blood lipid lowering extracts also had significant blood coagulation resisting activity.(4) Through layer chromatography separation and purification, total five polarity sections were obtained from Liubao tea ethyl acetate extracts. After utilizing the analysis column and general program to complete the gradient elution,2’tea with the best chromatographic separation condition was found out. Change the analysis column with the semi-preparation column, and further optimize the elution program of2’tea. At last,4purified peaks and their substances were derived by the best HPLC conditions. Adopting1H NMR and13C NMR as analytic method, the four peak substances were determined to be caffeine, gallic acid, rutin and quercetin. The result showed that these substances in Liubao tea ethyl acetate extracts might have significant functions, including blood lipid lowering and blood coagulation resisting. |
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