| Protein as the essential elements of life is closely related to human health.The study of protein can reveal the regularity of life activity,and it is also the basis for the diagnosis of many diseases However,matrix components often coexist with proteins in real samples.Therefore,the specific recognition and selective extraction of target proteins from complex systems is important for protein research.Molecularly imprinted polymers have specific recognition properties and have good stability which can resist certain environmental factors.Molecularly imprinted polymers have become the material for reliable identification,enrichment and separation.Molecularly imprinted technology combined with the fluorescence detection technology can effectively combine the advantages of specific recognition and sensitive detection.In this dissertation,different fluorescent molecularly imprinted polymers based on fluorescent organic nanoparticles,carbon dots and magnetic carbon dots as fluorescence sources were prepared for selective determination of bovine hemoglobin.The main results are as follows:1.The fluorescent organic nanoparticles based molecularly imprinted polymer(FONs@MIPs)were prepared by sol-gel method with fluorescent organic nanoparticles as fluorescence source,bovine hemoglobin as the template molecule,3-aminopropyltriethoxysilane as the functional monomer for highly selective fluorescence detection of bovine hemoglobin.The structure and component of the molecularly imprinted polymer was investigated by scanning electron microscopy,energy dispersive X-ray spectroscopy,and Fourier-transform infrared spectroscopy.The results showed that the molecularly imprinted polymer was successfully coated on the surface of the fluorescent organic nanoparticles.Under the optimum conditions,the fluorescence intensity of the FONs@MIP reduced gradually with the increment of BHb concentration range of 0.05 μM-8.0 μM,and the detection limit was calculated as 18.7 nM.Selective adsorption experiments showed that FONs@MIPs had high selectivity for BHb recognition in competitive proteins.2.A novel fluorescent imprinted polymer(CDs@MIP)with selective recognition of hemoglobin was prepared by the sol-gel method using fluorescent carbon dots as the carrier material,3-aminopropyltriethoxysilane as the functional monomer,tetraethoxysilane as the crosslinking agent and bovine hemoglobin as the template molecule.The results of IR and scanning electron microscopy showed that the molecularly imprinted polymer was coated on the surface of fluorescent carbon dots.The CDs@MIP showed selective recognition properties for bovine hemoglobin with an imprinting factor of 4.60.Its adsorption ability and specific recognition performance are discussed in detail.The results showed that the CDs@MIP had high selectivity toward bovine hemoglobin,and the selection factors for ovalbumin,bovine serum albumin and human serum albumin were 4.38,4.73,and 3.66,respectively.Under the optimal conditions,the linear range of CDs@MIP for bovine hemoglobin was 0.1-10.0 μM and the detection limit was calculated as 23.0 nM.The CDs@MIP was successfully used to determine bovine hemoglobin in bovine blood samples with recoveries of 99.3-104.0%.3.A simple and effective method was proposed to prepare an imprinted polymer layer at the surface of magnetic carbon dots with dopamine as the functional monomer for selectively and sensitively fluorescent recognizing bovine hemoglobin.The preparation conditions of M-CDs@MIP was optimized.The magnetic fluorescence imprinted polymers were investigated by Fourier-transform infrared spectra,scanning electron microscopy,energy dispersive X-ray spectroscopy,and vibrating sample magnetometer.Under optimum conditions,the fluorescent intensity decreased linearly coincided with the concentration of bovine hemoglobin in the range of 0.05 μM-16.0 μM with a detection limit of 17.3 nM.The M-CDs@MIP was used for detecting bovine hemoglobin in real samples with the recoveries of 99.0%-104.0%. |
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