Model Of Moving Substitution Reaction Boundary And The Stacking Method Derived From It

In this thesis, the development of capillary electrophoresis (CE) on-line sample preconcentration and the moving chemical reaction boundary (MCRB) were reviewed. As an on-line sample stacking technique, MCRB has been widely studied, and established a variety of MCRB models and the corresponding theory. The work of this paper is mainly focused on the establishment of the newly moving substitution boundary (MSB) model, the enrichment mechanism of the dynamic equilibrium system based on MSB and the verification of MSB model in lager gel-filled tube and in CE. The specific contents are as follows:1. The establishment of the newly moving substitution boundary (MSB) model. Based on the theory of MCRB, we put forward the newly MSB model, elaborated on the enrichment mechanism of the dynamic equilibrium system based on MSB, and discussed the variation of the boundary velocity and direction in different experiment conditions. And it is the first time to use a metal ion to stack another metal ion.2. The verification of MSB model in lager gel-filled tube experiment. In this chapter we verified various factors on the effect of the boundary velocity and the stacking efficiency of cobalt ion mentioned in the theoretical derivation of the second chapter. And the results indicated:(1) the actual existence of the dynamic equilibrium system composed of MSB and moving chelation boundary (MCB);(2) Cu2+had a key role in the stacking of Co2+in this study, a higher Cu2+concentration resulted in a better focusing efficiency;(3) the initial concentration of [Co-EDTA]2-and the initial length of [Co-EDTA]2-zone (Linitial) were the major factors on enrichment. A new type MCRB was successfully set up, and systematically expounded dynamic equilibrium system. This paper expanded the research on the field of MCRB and provided certain theoretical basis and experimental method for future metal ion enrichment mechanism.3. The verification of MSB model in CE. In this chapter, we investigated the effect of buffer, diffusion, the initial concentration of sample, the injection length of sample and the concentration and the injection length of copper ion on enrichment efficiency. The experimental results match with the third chapter, indicating that the applicability of the enrichment model in CE. Up to71-fold improvement in multiple of enrichment was achieved by using this enrichment model. This enrichment model of dynamic equilibrium system composed of MSB and MCB was successful used in CE for metal ion enrichment, as a new on-line sample stacking technique, enriched the on-line enrichment technology.