Construction Of Surface Plasmon Resonance System For Rapid Detection Of Dioxin

Dioxin is classified as one of the most toxic of a dangerous class of chemicals known as POPs (Persistent Organic Pollutants). In recent years, with the rapid development of society and economy, the speed of urbanization process is becoming faster, the generation and treatment capacity of solid waste and medical waste is becoming bigger and bigger. Among the solid waste disposal methods, the technology of waste incineration shows a rapid development momentum. According to the recently research, it is easy to bring about dioxin in the process of waste incineration. Traditional detection methods of dioxin-like compounds at present have their own disadvantages such as high cost, long detection period and complicated operations, which limit the short-term test of dioxin in a various industries. Therefore, it is of great significance for monitoring and controlling the dioxin to develop a rapid detection technology for dioxin which has the features of easy to use and low running cost.Firstly, the Surface Plasmon Resonance (SPR) bio-sensor system is introduced in the thesis. The bio-sensor system can monitor interaction between molecular, the dynamic process of reaction and the kinetic constant real time, has the feature of high sensitive、strong anti-interference ability、label free and small amount of sample. It has been used widely in the area of environmental monitoring. In the thesis, the system which was put up by laboratory is refined and improved. The model which was angle modulation is changed to the model which combines the angle modulation with intensity modulation together, the dislocation parallelogram optical system and multi-channel sample cell is developed, in addition, the area of software is improved and optimized.Secondly, in the thesis the features such as sensitivity and reproducibility of the system were tested by glucose sample solution and antigen-antibody reaction. The high concentration of glucose sample solution (0.11-0.55 mol/L) was tested in the way of angle modulation. The results showed that the concentration of glucose sample and the resonance angle met the basic linear relationship, and the linear relationship was ideal; the low concentration of glucose sample solution (3-7 mmol/L) was tested in the way of intensity modulation, the concentration of glucose sample and the intensity of reflected light met the basic linear relationship, too. The detection limit for glucoses sample was 0.18 g/l. In the process of antigen-antibody reaction, the results of dynamic and static were compared with each other and showed that the dynamic result was better to reflect the conditions of antigen-antibody binding process.On the basic of the previous experiments, the sample solutions of 2,3,7,8-TCDD were tested. Firstly, the conditions of the experiments which may affect the test results were optimized; Secondly, the sample solutions of 2,3,7,8-TCDD were detected by direct detection method and indirect detection method separately. The results of ELISA were compared with the results of indirect detection method. The finally results showed that the detection range of SPR bio-sensor system was 0.01-100 ng/mL, the IC50 was 0.355 ng/mL and the corresponding standard deviation was 0.00015. It is better to use the SPR technology for dioxin detection than ELISA in the area of features such as detection range, sensitivity and stability. In addition, a serious of sample solutions are tested by the two ways, and the results of the two technologies show a good linear relationship.