Automatically Leukocyte Sorting And γ-H2AX Staining On A Microfluidic Chip

Space radiation is one of the main factors in space missions causing organisms injury, and it is a serious threat to the safety of space flight. Studies of Radiation damage on living organisms in space radiation have become an important aspect for human exploration to the space environment. Therefore, it is necessary to rapidly and accurately diagnose and assess the injury caused by irradiation.It is reported that DNA double-strand breaks (DNA strand break site, DSBs) were induced by radiation. Also, y-H2AX has been used as a biomarker for detecting the radiation damage.In this paper, the level of the human peripheral blood lymphocytes y-H2AX expression by UVC radiation was analyzed by a flow cytometry. Results showed that a good dose-effect linear relationship was found in this treatment. Basing on the resistance pulse (RPS) and Labview program, the author used the PDMS-glass microfluidic chip to detect and sort erythrocytes and leukocytes from human peripheral blood automaticlly. Based on the previous results, the author automaticlly realized staining the HUVEC-C cells and detecting the fluorescence on the microfluidic chip to analysis the levels of y-H2AX expression.In conclusion, the author provided a new method for detecting and assessing on radiation damage in the early state. And it is meaningful for detecting radiation damage automaticlly and rapidly.