Polymer Microfluidic Chip Is Applied Research, Biological Analysis

The primary object of this thesis is to develop novel modification methods of the microfluidic chips substrates for the immobilization of biomolecules and the new-style of electrochemical detection, which will facilitate the research of proteomics and some other bio-analysis.Based on the review of the new progresses and important achievements on the fields of microfluidic chip and proteomics, we focused on the exploring of modification methods of two plastic materials for microchips, that is, poly methyl methacrylate(PMMA) and polyethylene terephthalate(PET), and then the realization of biomolecules immobilization in the above microchips. These strategies were applied in the analysis of proteomics and separation of enantiomers. A new-fashioned manner has been brought forward for the electrochemical detection of biomolecules in microfluidic system, whose feasibility has been testified by the analysis of amino acid, cysteine. The detailed points of this thesis are expanded as follows.1 Through the method of hot-embossing, PMMA microchip was fabricated for the later research. Chemical modification of poly(methyl methacrylate) microchannel surface has been explored to functionalize microfluidic chip systems. A craft copolymer was designed and synthesized to introduce the siliane functional groups onto the plastic surface firstly. Furthermore, through a silicon-oxygen-silicon bridge that formed by tethering to these functional groups, a stable patterning network of gel matrix could be achieved. Thus, anchorage of proteins could be realized onto the hydrophobic PMMA microchannels with bioactivity preserved as far as possible.2 The suggested scheme of immobilization method for biomecules was applied to the construction of enzyme proteolytic microreactor-on-chip. The proteolytic activity of immobilized trypsin has been demonstrated with the digestion products of cytochrome c and bovine serum albumin that were characterized using MALDI-TOF MS. The time required for the protelytic reaction is less than 5 s, which is the shortest one that has been reported till now. This research exhibited a simple but effective strategy of plastic microfluidic surface modification for protein immobilization in biological and proteomic research, which greatly shortened the analytical period and economized the consume at the same time.3 Employing the immobilization method demonstrated, bovine serum albumin(BSA) was introduced to the fabrication of chiral stationary phases(CSPs) by alumina sol-gel network...