Subcellular Localization And Response To Ca²⁺ And Glucose Treatment Of Arabidopsis CRK45

Plant receptor-like protein kinases (RLKs) play important roles in regulating plant development, hormone signaling, abiotic, and biotic stress responses in plants. In this study, the response of Arabidopsis protein kinase CRK45were analyzed under Ca2+and glucose treatment in wild type, the mutant crk45and CRK45-overexpression plants. At the same time, CRK45and YFP or GFP fusion vectors were constructed to detect the subcellular localization of CRK45. The main results are as follows:1. The homozygous knockout crk45mutant of T-DNA single copy insertion was obtained by gene-specific primer PCR amplification and RT-PCR identification.2. Real time PCR results showed that CRK45gene can be induced after CaCl2treatment in wild type, and the mutation of CRK45leads to increased sensitivity of CICBE to Ca2+.3. CRK45positively regulates glucose stress responses during germination, and CRK45gene can be induced by glucose in wild type.4. Two binary vectors p35S::CRK45-YFP and p35S::4-CRK45-GFP were constructed for genetic transformation, and homozygous transgenic plants have been obtained. The fluorescent proteins were observed in the cytoplasm in transgenic Arabidopsis by confocal laser scanning microscopy.