An NMR-based Metabonomic Study Of Gender-and Age-related Differences In KM Mice

The organism goes through changes and differences during aging started from birth to development, maturity and senium. These growing processes also affect the metabonomes of organism. It was reported that the changes of metabonomes related with different ages, genders and strains. However, previous work focused on changes mainly on biofluids at several time points in life, hence uncomprehensive. In this research, we chose Kunming mice, which was the special strain widely used in China to investigate the basic metabonome data during growth of Kunming mice and illustrate gender influences on the metabonomes of Kunming mice. Our research provides crucial information in minimizing metabolic background in the studies of metabolic process of drug intervention and disease processes.This thesis employed1H Nuclear Magnetic Resonance (NMR) spectroscopic techniques in conjunction with multivariate data analysis methods and carried out two aspects of metabonomic studies including genders and ages affecting the metabonomes of biofluids and organs such as urine, plasma, liver and kidney during71weeks of Kunming mice lives covering the process from birth, development, maturity and senium.This thesis contains four parts. First of all, the introduction, and in this part we mainly introduce the background information on current research of Kunming mice, on the development history and present status of metabonomics, such as, existing reports of age and gender factors influencing of the metabonomes, and the theories of Nuclear Magnetic Resonance (NMR) and multivariate data analysis methods. Second chapter introduces the experimental design, the process of sample collection, sample treatments, NMR detections, data analysis, analyzes and expands the effect of gender on metabonomes in the urine, plasma, liver and kidney extracts. Comparisons which were reported in gender between Kunming mice and other strains are also discussed here. We also report the mice weight and assignment of metabolites in samples. The third chapter explains the changes of metabonomes during the life time in Kunming mice. The last chapter concludes of the analysis, and points out the prospect of the future research.