| In this study, we investigated molecular classification and phylogenetic analyses ofScleractinian coral of Xuwen, using partial sequence of Ribosomal Large Subunitgene(nlsr DNA), cytochrome b gene(Cyt b), ATP Synthase subunit6gene(ATP-6). Theresults showed that:1) High G+C content was discovered, and transition rate which mainlyoccurred between A and G, was higher than transversion rate;2) The usage of codons waspartiality in Cyt b and ATP-6;3) The transition and transversion rates were higher in thesecond codon of Cytb, which accounted for46.3%and42.2%of total transition andtransversion rates, respectively;4) The frequence of transition was higher thantransversion, and mainly took place in the first loci of codon of ATP-6sequence;5)Scleractinia coral of Xuwen was mainly divided into two teams, one included Faviidae andFungiidae, and another included Agariciidae, Dendrophylliidae, Oculinidae, Poritidae,Acroporidae;6) Alveopora may be a undetected genus in Xuwen;7) Phylogenetic treebased on nlsr DNA suggested Oulastrea and Leptastrea should be classified intoAgariciidae.In addition we investigated molecular classification of Zooxanthellae, usingPCR-RFLP analyses of nuclear large-subunit ribosomal DNA(nLSU rDNA) and nuclearsmall-subunit ribosomal DNA(nSSU rDNA), and sequence analysis of internal transcribedspacers(ITS). Got the following results:1) The average contents between G+C(50.8%) andA+T(49.2%) were close. The transition rate being0.813%and transversion rate being0.315%indicated that there was less variation in the bases of ITS sequence;2) The62samples of Zooxanthellae from25species of scleractinia coral, which selected in15genusand8families of Xuwen, belonged to clade C, and could be further divided into twosub-clades for C1and C15.3) In the62samples, there were43samples symbiosis withC1, and30.65%symbiosis with C15;4) Only3corals in Xuwen had Symbiosispolymorphism, including Turbinaria irregularis, Turbinaria irregularis and Favitesabidita. |
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