The Construction Of Energy Transfer Model And The Reaserch On Phytochrome In Cyanobacteria

Photosynthesis is one of the most important chemical reactions in our world, and the energy lives need is provided by photosynthetic organisms in Photosynthesis-Synechocy-s tis sp. PCC6803is a useful tool in the study of photosynthesis, ApcE is a very significant linker protein in6803, and it play an important role in the enery transfer that from phycobilisome to PS Ⅱ. Hp49is a designed protein which has four repeated Alpha helixes, it can bind with various of pigment. While the Hp49(2a) which has only two Alpha helixes can also make it.In this paper, using E.coli expression we contrust two energy transfer models systems which named ApcE(1-240/△77-153)::Hp49(2a)::PCB::ZnMP and ApcE(1-240/△77-153)::Hp49(2a)::PEB:;ZnMP, and we verify that enery can transfer from Hp49(2a):: PCB to ApcE(1-240/△77-153)::PCB in the model of ApcE(1-240/△77-153)::Hp49(2a):: PCB::ZnMP by analysis of the absorption and fluorscence spectrum. While there is no enery transfer in ApcE(1-240/△77-153)::Hp49(2a)::PEB::ZnMP. And this result will make sense in our study in the enery absorption and transfer in Photosynthesis. Furthermore we get the△6803by replacing the cpcA::cpcB gene with the fusion gene which contains apcE and hp49.On the other hand, we also do some reaserch in bacterial phytochrome in Anabaena sp. PCC7120. We get gene all4261gaF2using molecular cloning technique, And the protein is studied by restructured with PCB. by analysis of the absorption and fluorscence spectrum we found that all4261GAF2::PCB has absorption at614nm and fluorescence at644nm. Througth the urea denature experiment we also get the ε of A114261GAF2::PCB,it is aboult31643M-1cm-1. And we also calculate the fluorescence quantum yield,it is about0.115.