Study On The Interaction Between Several Active Components From Natural Products And DNA

In the thesis, the binding modes between some natural products active components with calf thymus DNA were studied using different spectroscopic techniques, fluorescence, UV-vis absorption, circular dichroism, and Fourier transform infrared spectroscopy. The method of chemometrics in analysis of three-dimensional data also had been applied, which had obtained the pure spectra and equilibrium concentration of each component in complicated biochemical system.The thesis is comprised of five parts and the main conclusions are summarized as following.1. The biological characteristics of DNA was briefly stated. The action law, research method, and research status of interactions between small molecules with DNA were summarized either.2. The interaction between salidroside and DNA in physiological buffer (pH7.4) was investigated by fluorescence, and UV-vis spectroscopy, I-quenching effect, influence of ion strength, viscosity and DNA melting techniques.3. In physiological buffer solution, the interaction between farrerol and DNA was studied by fluorescence, UV-vis absorption, circular dichroism, and Fourier transform infrared spectroscopy. I-quenching effect, viscosity and DNA melting techniques. Neutral red (NR) was used as fluorescence probe. The effect of addtion of Cu2+, Co2+and Mg2+to the binary system was studied either.4. The interaction between paeoniflorin and DNA in physiological buffer (pH7.4) was investigated by fluorescence, UV-vis, and circular dichroism spectroscopy. Hoechst33258was used as fluorescence probe. Influence of addtion of Zn2+, Ca2+and Mg2+to the binary system was studied either.5. The interaction of rhein with DNA in pH7.4Tris-HCl buffer has been investigated by UV-vis, synchronous fluorescence and circular dichroism spectroscopy, viscosity and DNA melting techniques. Acridine orange (AO) was used as fluorescence probe.