Inhibition Of CaSR Expression In MDA-MB-231by SiRNA Interference

From ancient to present, breast cancer constitutes one of the daunting diseasesfor the health of the women worldwide. Breast cancer occurrence has been increasedas ageing of the population in the western countries. Recent years, occurrence of thisdisease is increasing too. Breast cancer has been a field of ongoing research with thedevelopment of the medical technology and innovation of the b iology field. CaSR(calcium sensing receptor) first cloned from calf parathyroid gland is a member ofG-protein coupled Family. Like all the others, it is a trans-membrane protein too.With N-terminal as the receptor and C-terminal as the activator, CaSR is proved to beimplicated in cell signal transmission. CaSR is a main factor for oncogenesis andmetastasis of kinds of cancers, like, breast, prostate, rectum cancer and so on. CaSR isexpressed both in benign and malignant breast tissues, but, in the highly metastaticbreast cancer cell lines the CaSR is highly expressed. The abnormal expression of theCaSR is an important factor for cancer cell oncogenesis, especially for theconsideration of breast cancer. RNA interference phenomenon is discovered by Firein1998. Now, the RNAi technology is used widely in the field of studying specificgene target and is an important way to researchers in biology technology, medical andpharmaceutical. Drugs used for targeting cancer cells by means of RNAi have been onthe market. In this experiment, we selected the useful and efficent small hairpin RNA.This vector has been transfected to breast cancer cell line MDA-MB-231, and itsefficiency was detected by Real-time PCR and Western-blot. This experiment hasproved that the vector we constructed was succeeded in silencing CaSR expression.