Cloning And Studies Of Phorbol Esters Biosynthetic Pathway Key Genes From Jatropha Curcas

Jatropha curcas is famous for biodiesel tree which contains various chemicals, such as diterpenoids, flavonoids, fats, proteins, and so on. Except extracted seed oil, Jatropha curcas could also be used in antibacterial, animal feeds, biomedicine, and soap manufacture.Phorbol ester is an important component in Jatropha curcas, which shows both toxicity and pharmacology activity to people. Research on the phorbol ester metabolic pathway could help us to understand how it was synthased in the plant, so that we might get a way to control its content in Jatropha curcas. Here we cloned several key genes of the phorbol ester biosynthase pathway.1) Farnesyl diphosphate synthase gene from J. curcasA novel cDNA encoding for farnesyl diphosphate synthase from J. curcas, named JcFPS was cloned by RACE, showing highly homologous to the FPSs of other species. The full-length cDNA of JcFPS was 1471 bp with 5’and 3’UTR, poly A tail and a 1026-bp ORF. The putative protein was 342 AA (amino acid) with a molecular weight of 39.45 kDa and a pⅠof 5.39.2) Phytoene synthase gene from J. curcas A novel cDNA encoding for phytoene synthase from J. curcas, named JcPSY was cloned by RACE, showing highly homologous to the PSYs of other species. The full-length cDNA of JcPSY was 1755 bp with 5’ and 3’ UTR, polyA tail and a 1287-bp ORF. The putative protein was 429 AA (amino acid) with a molecular weight of 48.07 kDa and a pⅠof 8.95.3) Squalene synthase gene from J. curcasA novel cDNA encoding for squalene synthase from J. curcas, named JcFPS was cloned by RACE, showing highly homologous to the SQSs of other species. The full-length cDNA of JcSQS was 1630 bp with 5’ and 3’ UTR, polyA tail and a 1239-bp ORF. The putative protein was 413 AA (amino acid) with a molecular weight of 47.24 kDa and a pⅠof 7.16.