| Background: Because of the limited source of allogeneic skin, Domestic andforeign scholars have been seeking to heterogeneous sources of acellular dermalmatrix (ADM)as a dermal treatment of deep skin damage, Due to a wide source andlow price, Pig source ADM become a hot topic of current research, Pig source ADMcan remain the three dimensional structure. But the slow revascularization limitingthe Pig source ADM widely used in clinical.Objective: To examine the biocompatibility of the Laser micropore porcineacellular dermal matrix (PLADM),and observed the revascularization rate after ittransplantation, Explore the possibility of the LPADM widely used in clinicalpractice.Methods:(1) cut full-thickness skin from the back of the adult SD rats, isolatedfibroblasts from it and cultured for the third generation, randomly divided into A, B,C group, Group A co-cultured with LPADM; group B co-cultured with non-porousADM; group C as pure culture group; observed the Situation of fiber cell growth,proliferation and detection the IL-6, IL-10,TGF-β1,VEGF,LN after inoculation1,3,5days use double sandwich ELISA(2) taking18adult male SD rats, doing two2cm×2cm∪-shaped flap in theback, respectively to transplant LPADM and non-porous ADM, after1,3,10-days cutspecimen for Histological and electron microscopic examination.(3)36male nude mice were randomly divided into experimental and controlgroups, use "two-step" complete the surgical procedure, the experimental grouptransplanted PLADM and autologous thin skin, the control group transplantednon-porous ADM and autologous thin skin, each group were taken to six nude micewere sacrificed in1,3,14days after surgery, cut specimens with HE histologicalexamination and electron microscopy.Results:(1) At Co-cultured conditions, LPADM and non-porous ADM can notproduce the secretion of cytotoxic substances affect fibroblast growth, proliferationand activity of cytokines, the fibroblasts of A, B, and C groups were able to adherent in15min after inoculation. The cell activity factor IL-6, IL-10of TGF-β1VEGFand LN no significant difference after inoculation, the difference was not significant(P <0.05).(2)In the collagen of LPADM can see the class of vascular endothelial cellsafter1day of Surgery,after3days,new tissue ingrowth in the micropore structure,after10days the LPADM can be closely integrated with the surrounding autologoustissue. During the whole experiment,Transplanted for non-porous ADM have noneovascularization, and severe inflammatory response in the experimentalobservation.(3) Histological sections and scanning electron microscopy shows that the newtissue which rich in blood vessels grow into the micropore structure,Thus completingthe rapid vascularization of LPADM. The presence of the micropore structure canalso provide a fast-track the fibroblasts to migrate in the LPADM, Provide the basisfor the reconstruction of the PLADM of collagen grid function. Scanning electronmicroscopy showed that the fibroblasts have a lot of rough endoplasmic reticulum,collagen secretion strong, and is actively involved in the repair of the dermis.Ofnon-porous ADM, we can not see fibroblasts move into collagen, and severeinflammatory response, and not combine with the body quickly and closely.Conclusion: The design of this LPADM is high biocompatibility; nocytotoxicity, low immunogenicity after transplantation. the micropore structure canprovide vascular channels for the LPADM, greatly accelerate the vascular speed ofthe LPADM. To solve the problem of slow clinically heterogeneous sources of ADMvascularization,provides the experimental basis of heterogeneous sources ADMwidely used in clinical... |
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