The Experimental Studies Of Repairment Of Skin Defects Utilizing Auto-KC And Allogeneic Acellular Dermal Matrix

The technique of skin tissue engineering has been a promising method to provide adequate skin grafts and improved wound healing quality. Various dermal analogs have been developed and used as dermal template to cover full-thickness wounds successfully. The dermal replacement grafted with thin autologous skin or cultured multilayer stratified epithelium could shorten healing time of donor sites. The recipient sites have improved cosmesis and function with less pigmentation, wound contraction and better durability. There are three parts in skin tissue engineering ,which include the sources of seed cells, the preparation of scaffold material of dermis and the additive of cytokine.The preparation of acellular dermal matrix is hot in scaffold material of skin tissue engineering.Several methods have been developed in excluding cells in dermis, but the shortcomings are obvious in some products.Based on the former studies, the novel method of NaOH-maceration was applied in preparation of acellular dermal matrix (ADM) for the first time. It was found that the structure of collagen fiber in the ADM was intact. The cytotoxicity of the ADM was scored as grade 1 ; The human fibroblasts seeded on the ADM could disperse into the natural pores and had the fine viabilities. The results of biocompatibility showed that the host cell could easily infiltrate in ADM and the vascularization could be observed. These results are according to the standard established by our national medical instrument department. The process of ADM preparation is simple, so there should be a broad clinic application in the future. At the same time, the methods of culturing and identifying the seed cells were studied. Our investigations will be of a great help to promote the use of tissue engineering in the repairment of skin defects.