| Objective：To observe the expressions of vasoactive intestinal peptide(VIP) proteins in thetissue of gastric carcinoma and normal gastric beside carcinoma, and CD74,MHC-II,CD80,CD86and CD40proteins in the inflammatory cells. To evaluate the relationsbetween VIP proteins in gastric carcinoma tissue and antigen presentationmolecules in the inflammatory cells.Methods：48patients that received a gastric cancer surgery from August2011to November2011at the First Affiliated Hospital of Nanchang University were enrolled in thecurrent study. Gastric carcinoma tissue, normal tissue peripheral to the carcinoma,and patient information were collected from each patient. Among the patients studied,36were male,12were female. The age of patients in this study ranges from34to85,with an average of59.4±11.2.24of the patient age from34to60, the other24wereabove60year old. Among these patients, the adenocarinoma of19cases located atthe pyloric antrum,29cases at the body of stomach,31cases had lymph nodemetastasis,17cases had no lymph node metastasis,25cases were highly ormoderately differentiated,23cases were poorly differentiated.18cases were in TNMstage I to II and30cases were in TNM stage III to VI. Immunohistochemical methodwas used to detect the expression of VIP protein in gastric carcinoma tissue and itsnormal peripheral tissue, and the expressional of CD74,MHC-II,CD80,CD86and CD40protein in the inflammatory cells.Results：1. The positive expression rates of VIP in gastric carcinoma tissue(94%) wassignificantly higher than its normal peripheral tissue(77%)(P<0.05). The expressionintensity of VIP in gastric carcinoma was significantly higher than its normalperipheral tissue (P<0.01).The VIP expression intensity in the atients with poorlydifferentiated degree, lymph node metastasis,or TNM III to IV, was significantly higher than the patients with well-moderately differentiated degree, no lymphnodemetastasis, or TNM I to II respectively(P<0.05). However the VIP expressionintensity does not change with respect to the sex, age, or cancer location within thebody(P>0.05)2. The positive expression rates of CD74in the inflammatory cells of gastriccarcinoma tissue(92%) was significantly higher than its normal peripheral tissue(73%)(P<0.05). The expression intensity of CD74in the inflammatory cells of gastriccarcinoma significantly was higher than its normal peripheral tissue (P<0.01).TheCD74expression intensity in the inflammatory cells of gastric carcinoma in theatients with poorly differentiated degree,lymph node metastasis,or TNM III to IV,wassignificantly higher than the patients with well-moderately differentiated degree, nolymphnode metastasis,or TNM I to II respectively (P<0.01). However the CD74expression intensity does not change with respect to the sex, age, or cancer locationwithin the body(P>0.05)3. The positive expression rates of MHC-II in the inflammatory cells of gastriccarcinoma tissue(38%) was significantly lower than its normal peripheral tissue(60%)(P<0.05).The expression intensity of MHC-II in the inflammatory cells of gastriccarcinoma was significantly lower than its normal peripheral tissue (P<0.01).TheMHC-II expression intensity in the inflammatory cells of gastric carcinoma in theatients with TNM III to IV was significantly lower than the patients with TNM I to II(P<0.01). However the MHC-II expression intensity does not change with respect tothe sex, age, cancer location within the body, differentiation groups, or lymph nodemetastasis (P>0.05).4. The positive expression rates of CD80in the inflammatory cells of gastriccarcinoma tissue(33%) was significantly lower than its normal peripheral tissue(60%)(P<0.01).The expression intensity of CD80in the inflammatory cells of gastriccarcinoma was significantly lower than its normal peripheral tissue (P<0.01).TheCD80expression intensity in the inflammatory cells of gastric carcinoma in theatients with lymph node metastasis,or TNM III to IV,was significantly lower than thepatients with no lymphnode metastasis,or TNM I to II respectively(P<0.05). Howeverthe CD80expression intensity does not change with respect to the sex, age, cancer ·location within the body, or differentiation groups (P>0.05)5. The positive expression rates of CD86in the inflammatory cells of gastriccarcinoma tissue(35%) was significantly lower than its normal peripheral tissue(60%)(P<0.05).The expression intensity of CD86in the inflammatory cells of gastriccarcinoma was significantly lower than its normal peripheral tissue (P<0.01).TheCD86expression intensity in the inflammatory cells of gastric carcinoma in theatients with TNM III to IV was significantly lower than the patients with TNM I to II(P<0.01). The CD86expression intensity in the inflammatory cells of gastriccarcinoma in the patients with poorly differentiated degree or lymph node metastasiswas lower than the patients with well-moderately differentiated degree or nolymphnode metastasis respectively (P>0.05). However the CD86expression intensitydoes not change with respect to the sex, age, or cancer location within the body(P>0.05)6. The positive expression rates of CD40in the inflammatory cells of gastriccarcinoma tissue(38%) was significantly lower than its normal peripheral tissue(60%)(P<0.05).The expression intensity of CD40in the inflammatory cells of gastriccarcinoma was significantly lower than its normal peripheral tissue (P<0.05).TheCD40expression intensity in the inflammatory cells of gastric carcinoma in theatients with lymph node metastasis,or TNM III to IV,was significantly lower than thepatients with no lymphnode metastasis,or TNM I to II respectively(P<0.01). Howeverthe CD40expression intensity does not change with respect to the sex, age, cancerlocation within the body, or differentiation groups (P>0.05)7. There was positive correlation between the expression of VIP in carcinomaand the expression of CD74in the inflammatory cells of gastric carcinoma. There wasnegative correlation between the expression of VIP in carcinoma and the expressionof MHC-II,CD80, CD86and,CD40in the inflammatory cells of gastric carcinoma.Conclusions：The expression intensity of VIP in gastric carcinoma was higher than the normaltissue peripheral to carcinoma. The expression intensity of CD74in the inflammatorycells of gastric carcinoma was higher than the normal tissue peripheral to carcinoma.The expression intensity of MHC-II,CD80,CD86,CD40in the inflammatory cells of gastric carcinoma was lower than the normal tissue peripheral to carcinoma. Therewere positive correlation between the expression of VIP in carcinoma and theexpression of CD74in the inflammatory cells of gastric carcinoma.There wasnegative correlation between the expression of VIP in carcinoma and the expressionof MHC-II,CD80,CD86,CD40in the inflammatory cells of gastric carcinoma. Duringthe process of gastic cancer development, VIP may inhibit the antigen presentationpathway to suppress the immune monitoring of gastric cancer and participate in theimmune evasion of gastric carcinoma. |
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