Pharmacodynamic Study Of H22Tumor-bearing Mice Transplanted Liver Cancer Model For Huazhengsanjikeli

Purpose: This topic using nude mice cancer strains passaged composite modelingmethod, the preparation of primary liver cancer mouse model.The liver, spleen index, livermorphological changes of serum ALT, ALP, and GGT content, the study of disease byobserving scattered plot particles learn H22tumor-bearing mice transplanted liver tumormodel efficacy. Lay the foundation for the development of high efficacy, high standard,high-quality anti-cancer liver protection drugs.Method: Preparation of transplanted H22hepatoma animal models, animals wererandomly divided into model group, of cantharides group and disease for particles scatteredplot, medium and high dose groups. Model group was given normal saline10ml/kg; thecantharides group dose1mg/kg; particles scattered plot of the disease, the administered dose,high dose group were:1.95g/kg,3.9g/kg,7.8g/kg.Continuous administration of water supplyof15days off the vertebral mice were sacrificed24hours after the last administration, theremoval of the eye blood, serum ALT, ALP, and GGT levels., The tumor tissue, tumor tissuesections, HE staining, detection of transplant H22tumor mice liver, spleen index, and observethe morphological changes of the liver in mice.Result: The experimental results show:①The of disease scattered plot particle dosegroup and spot beetle group of liver, spleen index and the model group compared there is asignificant difference (P <0.01); disease for bulk product particles of high-dose group of theliver, spleen index and modelgroup there was a significant difference (P <0.001).②ofdisease scattered plot particles in the high dose group of serum ALT, ALP, and GGT levelsdecreased significantly (P <0.01).③The results of HE staining: particles scattered plot ofdisease, the high dose group and cantharidin with the model group tumor foci decreased, andnormal tissue boundary more clearly.Conclusion: Particles scattered plot of disease, the high dose group and cantharidingroup can effectively inhibit the orthotopic liver cancer model of tumor growth in mice, andhepatoma cells induced apoptosis. Particles in the scattered plot of the disease, the high dosegroup inhibition of tumor growth by protecting the immune organs (such as the spleen),protect the liver cells directly, thus effectively enhance immune function.