Expression Of β-catenin And Axin And Correlation With Infiltration And Metastasis In Ovarian Cancer

Objective：Ovarian cancer is a common malignant tumor of womenreproductive system, its morbidity is the second in gynecology malignanttumor, however, its mortality rate is the first. Because of growth mode andlocation of ovarian cancer, the early symptoms are not characteristic. Due toovarian carcinoma developed easily and transfered widely, so about70%~80%patients are often already advanced ovarian carcinoma when seeinga doctor。Some research show that Wnt signalling transduction pathway playsan important role in incidence,development,infiltration and metastasis oftumor。The change of such pathway plays an important role in incidence anddevelopment of ovarian carcinoma. β-catenin is one of the most componentsin the Wnt signal pathway and the stabilization is important to such pathway.Axin is an important negative adjustment molecular in Wnt/β-cateninsignalling transduction pathway. Some research show that there is a closeconnection between most of tumors and the mutation of Axin.In this study, we discuss the protenin expression level and relationship ofβ-catenin and Axin in ovarian cancer and ovarian benign tumor tissue. Therelation was analyzed among the protein expression with FIGO stage,histological classification, cell differentiation.To realize the mechanism offormation process in cancer.We hope they can make certain base for thetherapy of epithelial ovarian caner.Methods:1The choice of specimen：Altogether55pieces of pathological specimenfrom epithelial ovarian cancer and ovarian benign tumor tissue (with15casesof ovarian benign tumor tissue and40cases of epithelial ovarian cancer)inThe Four hospital,Hebei Medical University. All the cases of epithelialovarian cancer was not treated by anti-cancer therapy. 2With No. for all specimens, It was fixed by10%formalin. The specimenthat fit the standard was embedded by paraffin. All of them were made into4μm slices. Immunohistochemistry was used to detect the expression statusofβ-catenin and Axin in40cases of epithelial ovarian cancer and15cases ofovarian benign tumor tissue.3Statistics: After datas were collected, SPSS13.0was applied to analyzethe results of experiment. To analyze expression difference of two kinds ofproteins in epithelial cancer tissues and ovarian benign tumor tissues, andrelationship with FIGO stage, histological classifycation, cell differentiation.Results:1Immunohistochemistry results showed that the positive staining ofβ-catenin protein occupied46.7%(7/15) in ovarian benign tumor tissues andfor40cases of epithelial ovarian cancer specimen, positive staining ofβ-catenin protein occupied77.5%(31/40). The expression rate of β-catenin inovarian benign tumor tissue was lower than that in the epithelial ovariancancer tissue, there was very significant difference between the two groups(P<0.05). For ovarian benign tumor tissue, positive staining of Axin proteinoccupied80.0%(12/15)and the positive expression rate of Axin protein was47.5%(19/40) in epithelial ovarian cancer, there was obvious differencebetween groups(P<0.05).2The positive expression rate of β-catenin gene protein in high-moderatedifferentiation cancer was lower than that of low differentiation.Thedifference was significant(P<0.05). The expression ofβ-catenin gene proteinin early cancer (Ⅰ, Ⅱ stage ovarian cancer) was significantly lower than thatin Late-stage cancer (Ⅲ, Ⅳ stage ovarian cancer), The difference wassignificant (P <0.05)；In different histological classifycation of ovarian cancerβ-catenin gene expression was no obvious difference(P＞0.05).3The positive expression rate of Axin gene protein in high-moderatedifferentiation cancer was higher than that of low differentiation.Thedifference was significant(P<0.05). The expression of Axin gene protein inearly cancer (Ⅰ, Ⅱ stage ovarian cancer) was significantly higher than that in Late-stage cancer (Ⅲ, Ⅳ stage ovarian cancer), The difference wassignificant (P <0.05)；In different histological classifycation of ovarian cancer,Axin gene expression was no obvious difference(P＞0.05).4There was negative correlation between β-catenin and Axinexpression(r=-0.447, P=0.001, P <0.05).Conclusions:1The aberrant expression of β-catenin in epithelial ovarian cancer wasobviously higher than that in ovarian benign tumor tissue,that showtheβ-catenin might be promote the invasion and metastasis of epithelialovarian cancer. The normal expression of Axin in epithelial ovarian cancerwas obviously lower than that in ovarian benign tumor tissue which indicatedthe Axin could inhibit the invasion and metastasis of epithelial ovarian cancer.2The positive expression rate of β-catenin gene protein in high-moderatedifferentiation cancer was lower than that of low differentiation,the differencewas significant(P<0.05). The expression ofβ-catenin gene protein in earlycancer was significantly lower than that in Late-stage cancer, the differencewas significant (P <0.05)；The positive expression rate of Axin gene protein inhigh-moderate differentiation cancer was higher than that of lowdifferentiation,the difference was significant(P<0.05). The expression ofAxin gene protein in early cancer was significantly higher than that inLate-stage cancer, the difference was significant (P <0.05)；In differenthistological classifycation of ovarian cancer,β-catenin and Axin geneexpression was no obvious difference(P＞0.05).3The expression of β-catenin and Axin was negative correlation in epithelialovarian cancer tissues.