Research Of The Relationship Between The Gene Polymorphism Of POU4F3and Noise-induced Hearing Loss In Military

【Objectives】 To preliminary grasp the relationship between the gene polymorphism of POU4F3and noise-induced hearing loss in military via the research of the relationship between detection of gene mutation in the artillery, tank soldiers and armored force in the Yunnan province military area and noise-induced hearing loss. So that provides an efficient approach to prevent the explosive deafness,and makes contributions to the army medical career and other noise-concerned industries.【Methods】Taking the informed artillery, tank soldiers and armored force who are normal in hearing before military training but suffer the hearing loss after military operation in the southwestern military area as the object, to take96soldiers'peripheral venous blood,61are in experimental group,35are in matched group. Through the collection cubits venous blood2ml, EDTA anti-coagulation, extract DNA, polymerase chain reaction (PCR) POU4F3gene amplification on purpose extract, product direct gene sequencing and gene sequence analysis, the author found that there are two samples in which rs121909056single nucleotide sites have mutations In POU4F3genes in the experimental group, there is no mutations in the matched group.【Results】In the blood samples of61patients in the experimental group, two suspects whose rs121909056single nucleotide sites have mutations In POU4F3genes; from G→T, there is no mutations in the matched group. Through the test X2, the author got X2=1.1719, P=0.279>0.05, there is no obvious statistical meaning in the difference of this two groups.【Conclusion】The research results indicate that noise has the obvious effect to hearing, and there is Individual susceptibility difference. POU4F3genes are the hot genes in genetic deafness. The two suspects whose rs121909056single nucleotide sites have mutations In POU4F3genes are often exposed to the noisy environment, which are in accordance to the speculation that such mutations is conditions pathogenic mutations, but no obvious correlation to explosive deafness.