MYD88Expression And Mutation Detection In Colorectal Cancer

Objective:Detecting the mRNA expression level and hot mutations of the myeloid differentiation primary response gene88(MYD88)in colorectal cancer. Exploring linkage between MYD88and colorectal cancer to develop its clinical significance.Methods:MYD88mRNA expression level was detected in72pair of cancer and normal tissues by real-time fluorescent quantitative PCR (RT-PCR). MYD88gene mutation L265P was detected in111cases of colorectal cancer by DNA sequencing. Expression datas combined with clinical and pathological information were analyzed. The specimens were confirmed by pathology of colorectal cancer or colorectal normal tissue.Result:1.Relative MYD88expression levels of cancer tissues and adjacent normal tissues were10.112±1.511and15.010±1.1.977. Eexpression level of MYD88in cancer tissue significantly lower than in adjacent normal tissue (p<0.05). MYD88expression level in cancer tissues may not determined by clinicopathological parameters (gender, age, degree of differentiation, tumor size, CUA, CAI19-9, with or without lymph node metastasis, distant metastasis, depth of invasion, the distribution of parts, the Duke's stage)(p>0.05)2.MYD88mutant L265P do not exist in our111colorectal cancer tissuesConclusion:MYD88low expression plays an important role in the development of colorectal cancer, both may be wireless sexual relations. MYD88expression has nothing to do with clinical pathological parameters. No MYD88mutations were detected in colorectal cancer tissues. Therefore, considering that MYD88present lower expression level in CRC tumor tissues, it is expected to become a new target for tumor diagnosis and treatment.