The Effects Of Postoperative Analgesia With Ketorolac On The Levels Of IL-6and IL-10in Rats

Objective:This study through to establish the rat model of postoperative analgesia, observing the pro-inflammatory cytokines IL-6and anti-inflammatory cytokine IL-10after surgery at different phases and monitoring the dynamic expression of the two factors in the spleen immune organ, we in order to investigate the influence on body's immune function of postoperative analgesia, and provide a theoretical and experimental basis for clinical.Methods:75Wistar rats, male or female, were randomly divided into control group (D)25, the surgical model group (S)25and ketorolac Tromethamine treatment group (T)25. Each group was further divided into3,6,12,24,48hours five subgroups, each subgroup n=5. The rats of group D to be2%sodium pentobarbital anesthesia only. The rats of group D were shaved after anesthesia, and4-cm dorsal midline incisions were made over their thoracic spines. The wounds were then irrigated and closed using an interrupted5-0nylon suture. The rats of group T were handled on the basis of the S group after30minutes, and were given ketorolac Tromethamine5mg/kg intramuscularly every6hours for24hours. All postoperative rats could free access to rat chow and water. The animals of Group D, S and T were sacrificed by2%sodium pentobarbital after postoperative3,6,12,24,48hours, and were extracted the portal vein blood samples at room temperature for15minutes. At first, the samples were centrifuged3000r/min for10min, and then centrifuged3000r/min for10min, at last, serum was stored at-20℃. The expression of interleukin-6and interleukin-10in spleen tissue was tested by Real-time PCR. ELISA was employed to detect the level of interleukin-6and interleukin-10in blood-serum.Results:(1) In control group, IL-6, IL-10in serum and spleen tissues have no significant change within48hours.(2) IL-6in serum and tissue has raised at3hours, peaked at24hours, and then decreased significantly at48hours. There was a remarkable higher level of interleukin-6in S group compared to D group at12h and24h (P<0.01).(3) IL-10in serum and tissues also began to increase at3hours, continued to rise at the later9 hours, and then decreased at24h. In S group, IL-6in serum increased significantly at6h (P<0.05), and they had an obvious difference (P<0.01). But there were significant differences only at12h between the S and T group (P＜0.05).(4) In group T, IL-6or IL-10both in serum and tissues were lower than the S group in the corresponding time.Conclusions:(1) The rats of non-postoperative analgesia existed pro-inflammatory cytokine IL-6and anti-inflammatory cytokine IL-10"high level balance" state.(2) Compared with the S group, the T group cut down the concentration of IL-6, IL-10both in serum and tissues, and it indicates that ketorolac may could lower the "high level balance" of pro-inflammatory cytokine and anti-inflammatory cytokine in rats after surgery.(3) The IL-6and IL-10in rat serum had the same change as the tissues. Monitoring the level of IL-6and IL-10cytokine in serum could basically reflect the expression of IL-6mRNA and IL-lOmRNA in the spleen tissue, so it can be used as indicators of immune function in postoperative analgesia rats.