Hemostasis Effect And Histocompatibility Observation Of The Residual Cavity After Lobe Resection With Foley Catheter Balloon Tamponade

Objective: To observe hemostatic effect and tissue compatibility of theresidual cavity after lobe resection with Foley catheter balloon tamponade. Soit can provid preliminary experimental evidence for clinical application andfind out simple methods for the organizations compatibility of intracerebralgraft.Materials and Methods:1. Operation:1.1Experimental animals: There are eighteen New Zealand whiterabbits (All are3-month-old). Weight of2000±200g. Half male and female.Randomly divide into two groups(nine rabbits of each group). Give normaldiet and feed in rabbit cage.1.2Operation: After disinfection and anesthesia, exsect some braintissue of experimental group animals, and then place the balloon in theresidual cavity. Equally, exsect some brain tissue of control group animals,but don't place balloon.1.3Post-operative processing: We deal with the brain tissue of twogroups by hematoxylin and eosin staining(HE staining), Masson staining,CD68immunohistochemisty, Nissl body staining, silver staining,B-modeultrasonic, and animal behavior observed. Then compare the two groups inthese aspect. Each group were randomly divided into three subgroups: afterone day, after two days, after three days. Three rabbits of every subgroup.2. Observe animal behavior after surgery:Observe neurologic dysfunction, abnormal mental and eating activities ofthe postoperative animal.3. Histocompatibility observation: 3.1Methods: HE staining, MASSON staining, CD68immunohistochemistry, Nissl body staining, silver staining.3.2Section observation: Use the microscope to observe inflammatorycell infiltration of brain tissue, the pathological changes of neuronal cellbodies and axons, migration of the microglia and macrophage.4. Hemostatic effect observation:4.1B-mode ultrasound: To observe the operation area after1day,2days,3days under the B-mode ultrasound, and then contrast.4.2Section observation: Observe the number of red blood cells andbrain tissue matrix loosening degree by Masson staining under the microscope.Results:1. Comparison of the two group cells: there was no significant differencein Macrophages of the two groups; no significant difference in granulocyteand lymphocytes of the two groups either.(From the HE staining)2. Comparison of two group tissue: The first day to the third day, thebrain tissue matrix gradually became loosened, the red blood cellsoverflowing increased day by day, and no blood vessel was visible.(FromMasson staining)3. Compare red blood cells of the two groups, the experimental groupwas better than the control group, and there was statistical significance.4. CD68immunohistochemistry comparison of two groups: There was nosignificant difference.5. B-mode ultrasound photos comparison of two groups: The liquidanechoic area of experimental group was lighter then the control group.6. Compare the subgroups in each group: Brain tissue atrix graduallybecame loosening.Red blood cell overflowing,macrophages and granulocyteswere increasing day by day. Axons gradually swelled and disaggregated(From silver staining). Degenerated and apoptosis Neuronal cell becamemore.(From Nissl body staining)7. Reference to The Banff2007working classification of skin-containingcomposite tissue allograft pathology: The first day, grade0-II. The second day, grade II-III. The third day, grade III-IV.8. Reference to the People's Republic of China national standard GB/T-14233.2.93Infusion, transfusion, injection equipment testing methods:Inflammatory cell reaction grade I---IV of class. Generally, there is anupward trend.Conclusions:1. The Foley catheter has good histocompatibility.2. Balloon tamponade is effective on the hemostatic aspect.3. Initially provide a simple compatibility test for brain grafts.