| Objective:Observing the influence of mild moxibustion and specific electromag-netic spectrum Therapeutic Apparatus (TDP) on refractory wound rats'Healing time, Wound healing rate, IL-6, IGF-1, macrophage cell of the surface,MVD,col-Ⅰ and col-Ⅲ. Studying the effect of mild moxibustion on repairingWound tissue,then to provide experimental bases for Refractory woundtreatment of simple obesity.Methods:Duplicated rat refractory wound model by injecting Hydrocortisonesodium succinate under the skin of legs. Selected born6weeks, weight200～220g of healthy SD male112rats, They were randomly divided into twogroups, the first batch were32rats that mainly used to observe the differenttreatments on wound healing rate and wound healing time, Intraperitonealinjection of ketamine hydrochloride to150mg/kg, anesthesia modeling area(middle to upper lumbar spine) shearing, using plastic bottle with a diameterof20mm mark in the modeling area, disinfecting with Bromo Geramine alongthe Subcutaneous tissue to fascia tag line cut, bleeding, covering the woundwith sterile gauze dressing, and they wera randomly divided into4groups:theblank TDP Group (hereafter referred to as the blank group), mild moxibustiontreatment group (hereinafter referred to as the Moxibustion Group), TDPTherapeutic Apparatus in the treatment group (hereinafter referred to as theTDP Group) and model, every group were8rats,a separate sub-rearing cage;The second batch were80rats that mainly observed theserum IL-6levels atthird day after four model duplicated. They were randomly divided into4groups: normal TDP Group (hereafter referred to as the normal group),Moxibustion Group and TDP Group and model, normal group were8rats, The other groups were24rats in each,The rats in the Moxibustion Group, TDPGroup and model group used the same modeling approach.Kept separatelysharing the cage in each group.Since modeling the next day, the rats inMoxibustion Group, TDP Group and model group were injected hydrocor-tisone sodium succinate100mg/kg,1time every other day, they were injected7times. At the same time the treatment was started,the rats in the MoxibustionGroup were placed in moxibustion box that was made for treating, and thenmild moxibustion with a moxa treated local wound and bilateral Shenshu,Zusanli each15min, and the rats in the TDP Group using TDP treated15minby the same way, the rats in model group, normal group and blank groupwereplaced in moxibustion boxes15min, but not for other therapy. The rats of eachgroup were freely eating and drinking during the treatment, ordinary rat baitvessel from Hebei Medical University experimental animal center during theacupuncture intervention.The wound healing time of each experimental group were recorded byspecial messenger after intervention, and the wound area were sheet using oneCamera in fixed height plane by special messenger, then made by computer.Examined IL-6at the second day after model duplicated, and examined IGF-1,local organizations, CD34expression, MVD, Col-Ⅰand Col-Ⅲ for differentexperimental at the third day or seventh day or tenth day or fourteenth dayafter model duplicated.All the data were made statistics analysis with SPSS13.0software.Result:1Moxibustion methods on healing timeAfter treatment, the wound healing time (days) of Moxibustion Group,TDP Group, model group (18.20±1.48,21.40±2.07,25.40±1.51) werelonger than blank group (15.20±1.30) more than3days (P <0.01), show thatthe model successfully. And Moxibustion Group, TDP Group and model group,the differences among the three groups were statistically significant (P <0.01).2IL-6levels (pg/ml) at second day after duplicatedAt third day after model duplicated, IL-6levels of Moxibustion Group, TDP Group, blank group(80.77±4.68,80.29±4.68,77.56±7.62) werehighter model group(76.38±6.04), but the differences were not statisticallysignificant(P>0.05), and Moxibustion Group, TDP Group and blank group,the differences among the three groups were not statistically significant(P>0.05), which showed that the rats in4groups were damaged had no signi-ficant difference.3Moxibustion methods on the wound healing rate(%) at different time3.1Moxibustion methods on the wound healing rate(%) at the third dayafter model duplicatedAt third day after model duplicated, the wound healing rate(%) of blankgroup, Moxibustion Group (19.71±2.06,18.86±1.68) were highter thanmodel group(16.86±1.57)more than2%(P<0.05), but TDP Group (18.14±1.35) was highter than model group more than1.3%(P>0.05). And Moxibu-stion Group, TDP Group and blank group, the differences among the threegroups were not statistically significant(P>0.05).3.2Moxibustion methods on the wound healing rate(%) at the seventhday after model duplicatedAt seventh day after model duplicated, the wound healing rate(%) ofMoxibustion Group, TDP Group, model group(54.00±3.74,48.14±4.49,36.43±2.37) were lower than blank group(63.71±2.87) more than9%(P<0.05), And Moxibustion Group, TDP Group and Blank group, the differe-nces among the three groups were statistically significant (P <0.05).3.3Moxibustion methods on the wound healing rate(%) at the tenth dayafter model duplicatedAt tenth day after model duplicated, the wound healing rate(%) ofMoxibustion Group, TDP Group, model group(66.43±3.1,57.29±2.14,44.29±2.93) were lower than blank group(80.57±3.26) more than14%(P<0.05), And Moxibustion Group, TDP Group and model group, the differe-nces among the three groups were statistically significant (P <0.05).3.4Moxibustion methods on the wound healing rate(%) at the fourteenthday after model duplicated At fourteenth day after model duplicated, the wound healing rate(%) ofMoxibustion Group, TDP Group, model group(86.43±3.45,76.29±5.41,60.0±2.77) were lower than blank group(95.29±2.56) more than9%(P<0.05), And Moxibustion Group, TDP Group and model group, the differe-nces among the three groups were statistically significant (P <0.05).4Moxibustion methods on IGF-1levels (ng/ml) at different time4.1Moxibustion methods on IGF-1levels (ng/ml) at the seventh day aftermodel duplicatedAt seventh day after model duplicated, the IGF-1levels (ng/ml)of TDPGroup, model group, normol group(40.72±10.20,40.39±6.91,36.17±8.71)were lower than Moxibustion Group (55.43±12.78) more than14ng/ml(P<0.05), but TDP Group, model group and normol group, the differencesamong the three groups were not statistically significant(P>0.05).4.2Moxibustion methods on IGF-1levels (ng/ml) at the tenth day aftermodel duplicatedAt tenth day after model duplicated, the IGF-1levels (ng/ml)of TDPGroup, model group, normol group(119.46±47.69,60.37±5.84,36.17±8.71)were lower than Moxibustion Group (324.16±72.22) more than205ng/ml(P<0.01), and the TDP Group and model group, the normal differencesbetween the groups were statistically significant (P<0.05), between modelgroup and normal group were statistically significant difference (P<0.05)4.3Moxibustion methods on IGF-1levels (ng/ml) at the fourteenth dayafter model duplicatedAt fourteenth day after model duplicated, the IGF-1levels (ng/ml)ofmoxibustion Group, TDP Group, model group(89.68±10.43,99.43±12.17,108.25±19.78)were more than normol group (36.17±8.71) more than53ng/ml(P<0.01), and the moxibustion Group and TDP Group,model groupdifferences between the groups were statistically significant (P<0.05), betwe-en TDP group and model group were statistically significant difference (P<0.05)5Moxibustion methods on wound macrophage content(a/mm~2) at different time5.1Moxibustion methods on wound macrophage content(a/mm~2) at theseventh day after model duplicatedAt seventh day after model duplicated, the average wound macrophagecontent(a) in one view of Moxibustion Group, TDP Group, model group(10.0±0.76,9.25±1.17,6.00±0.76) were more than normol group(4.50±1.20)1.5or more(P<0.05), and between Moxibustion Group and model group werestatistically significant(P<0.05), but between Moxibustion Group and TDPGroup were not statistically significant(P>0.05).5.2Moxibustion methods on wound macrophage content(a/mm~2) at thetenth day after model duplicatedAt tenth day after model duplicated, the average wound macrophagecontent(a) in one view of Moxibustion Group, TDP Group, model group(25.00±2.93,21.75±1.58,16.75±1.91) were more than normol group(4.50±1.20)12or more(P<0.01), and Moxibustion Group, TDP Group and model group,the differences among the three groups were statistically significant (P <0.05).5.3Moxibustion methods on wound macrophage content(a/mm~2) at thefourteenth day after model duplicatedAt fourteenth day after model duplicated, the average wound macrophagecontent(a) in one view of Moxibustion Group, TDP Group, model group(11.75±1.20,15.00±1.31,18.25±1.58) were more than normol group(4.50±1.20)7or more(P<0.01), and Moxibustion Group, TDP Group and model group, thedifferences among the three groups were statistically significant (P <0.05).6Moxibustion methods on wound MVD(a/cm~2) at different time6.1Moxibustion methods on wound MVD(a/cm~2) at the seventh day aftermodel duplicatedAt seventh day after model duplicated, the average wound MVD(a/cm~2)of Moxibustion Group, TDP Group, model group(12.80±1.30,15.60±1.34,14.60±1.14) were more than normol group(10.80±1.48)2.8or more(P<0.01), Moxibustion Group, TDP Group and model group, the differencesamong the three groups were statistically significant(P <0.05orP <0.05), between Moxibustion Group and TDP Group, the differences were not statist-ically significant(P>0.05).6.2Moxibustion methods on wound MVD(a/cm~2) at the tenth day aftermodel duplicatedAt tenth day after model duplicated, the average wound MVD(a/cm~2) ofMoxibustion Group, TDP Group, model group(22.20±1.64,19.60±1.14,16.20±1.30) were more than normol group(10.80±1.48)5or more(P<0.01),and Moxibustion Group, TDP Group and model group, the differences amongthe three groups were statistically significant(P<0.01).6.3Moxibustion methods on wound MVD(a/cm~2) at the fourteenth dayafter model duplicatedAt fourteenth day after model duplicated, the average wound MVD(a/cm~2)of Moxibustion Group, TDP Group, model group(12.40±1.13,14.50±1.24,19.30±1.67) were more than normol group(10.80±1.48)1.6or more(P<0.01),and Moxibustion Group, TDP Group and model group, the differences amongthe three groups were statistically significant(P<0.05or P<0.01).7Moxibustion methods on wound COL-Ⅰ,COL-Ⅲ at different time7.1Moxibustion methods on wound COL-Ⅰat the seventh day aftermodel duplicatedAt seventh day after model duplicated, the average light density ofwound COL-Ⅰof Moxibustion Group, TDP Group (0.075±0.116,0.064±0.01)were highter than model group(0.035±0.061) more than0.02(P<0.01),but between Moxibustion Group and TDP Group were not statistically signifi-cant(P>0.05).7.2Moxibustion methods on wound COL-Ⅲ at the seventh day aftermodel duplicatedAt seventh day after model duplicated, the average light density ofwound COL-Ⅲ of Moxibustion Group, TDP Group (0.070±0.009,0.063±0.007) were highter than model group(0.031±0.009) more than0.02(P<0.01),but between Moxibustion Group and TDP Group were not statistically signifi-cant(P>0.05). 7.3Moxibustion methods on wound COL-Ⅰat the fourteenth day aftermodel duplicatedAt foueteenth day after model duplicated, the average light density ofwound COL-Ⅰof Moxibustion Group, TDP Group (0.182±0.116,0.119±0.030were highter than model group(0.075±0.006) more than0.04(P<0.01),and between Moxibustion Group and TDP Group were statistically significant(P <0.01).7.4Moxibustion methods on wound COL-Ⅲ at the fourteenth day aftermodel duplicatedAt foueteenth day after model duplicated, the average light density ofwound COL-Ⅲ of Moxibustion Group,TDP Group (0.188±0.356,0.120±0.316)were highter than model group(0.065±0.009) more than0.05(P<0.01),and between Moxibustion Group and TDP Group were statistically signifi-cant(P <0.01).7.5Moxibustion methods on wound COL-Ⅰ/COL-Ⅲ at the seventh,fourteenth day after model duplicatedAt seventh day after modeling, the rats with normal wound Ⅰ/Ⅲratio(0.97±0.159) compared, model group (1.23±0.351) difference was statisti-cally significant (P<0.05), moxibustion group and TDP group (1.09±0.247,1.02±0.239) difference was not statistically significant (P>0.05), moxibustiongroup and model group, the difference insignificant (P>0.05), TDP group andmodel group, the difference was statistically significant (P<0.05); At fou-teenth day after modeling, compared with normal group, model group (1.20±0.270) was significantly (P<0.05), moxibustion group (1.02±0.231) and theTDP group (0.87±0.217) difference was not statistically significant (P>0.05),moxibustion group and the TDP group and model group, the difference wasstatistically significant (P<0.01orP<0.05), moxibustion group and the TDPgroup care, the difference was statistically significant (P<0.05).Conclusion:1Moxibustion and TDP all could significantly shorten the healing time,but moxibustion was better. 2Moxibustion and TDP can be controlled in different periods of therefractory wound serum IGF-1levels. Early release of IGF-1activity byregulating platelet and fibroblast, increase its serum levels, to stimulateendothelial cell migration to the trauma site, prompting the formation of newblood vessels, and then to promote wound healing; late but also reduce theserum content, promote the excessive proliferation of collagen degradation,and reduce scar formation.3Moxibustion and TDP can adjust the number of different times in ratswith refractory wound macrophages. Early can improve wound macrophagesto promote phagocytosis and clearing of foreign body and aging injuries cellfunction, at the same time through the secretion of a variety of biologicallyactive substances to stimulate tissue angiogenesis and promote wound healing;late but also to reduce the number of wound macrophages, highlighting therole of regulating the synthesis of connective tissue matrix and decomposition,and thus make the collagen dissolved repeated deposition and update to tissueremodeling, reducing scar the purpose.4Moxibustion and TDP can be controlled in rat dermal wound MVD indifferent periods. Can improve wound MVD in early to improve the localmicrocirculation, prompting the damaged tissue to be repaired; late can bereduced to the wound MVD avoid scar tissue hyperplasia, and improving therepair quality.5Moxibustion and TDP could regulate the col-Ⅰ, col-Ⅲ on refractorywound, through regulating the activity of fibroblasts, promoted col-Ⅰ,col-Ⅲsynthesis and secretion, and then the wounds are repaired, and could adjustⅠ/Ⅲ to reducing scar formation in the healing of wounds.6Moxibustion and TDP could promote wound healing, but the timelinesswas different, mild moxibustion played more faster, and effect was moredurable, the overall treatment effect was better than TDP. |
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