Study On The Association Of VEGF Genetic Polymorphisms With Lung Cancer

Objective:Lung cancer is the most common malignancy,accountingfor about one fifth of all cancer mortality, morbidity and mortality wererapid upward year by year. According to the information provided by theOffice of the National Cancer, lung cancer mortality in China in the1970sas5.47/one hundred thousand, came in the esophagus, liver, stomach andcervical cancer after lung cancer mortality in China in the1990s, around17.27/one hundred thousand of Health in the21st century launched thethird cause of death review investigation, the lung cancer mortality wasincreased. The research shows that smoking, air pollution, occupationaldust exposure, lifestyle, bad and so many factors will affect the occurrenceof lung cancer, epidemiological studies have demonstrated that smokingcan significantly increase morbidity and mortality of lung cancer. With thecontinuous progress of the molecular epidemiology of lung cancer, lungcancer cell and molecular biology level, the study on lung cancer geneticsusceptibility to the direction of gradually attention has been paid.Vascular endothelial growth factor (VEGF) regulate angiogenesisregulatory factor, its receptor binding after both increased vascularpermeability, and promote the proliferation of vascular endothelial growth,eventually leading to the proliferation and metastasis of the tumor.Tumorgrowth is attributed to its unlimited proliferation, and metastasis ofmalignant potential, and angiogenesis. To provide oxygen and nutrients, isalso a key to tumor growth of tumor blood vessels to generate anunderstanding of the occurrence of malignant tumors, the development ofbiological characteristics and mechanism of invasion and metastasis ofmany important theoretical significance and clinical value.This studyaimed to investigate the VEGF gene in the promoter region of the-460T/ C,+405G/C single nucleotide polymorphism (single nucleotidepolymorphisms, SNPs) and lung cancer susceptibility of Chinese Hanpopulation, and further elucidate the molecular biology of lungcancermechanism.Methods: This study comprised260patients with lung cancer and260frequency-matched healthy controls with no evidence of disease from.Five milliliter of venous blood from each subject was drawn in Vacutainertubes containing EDTA and stored at4℃, Genotypes of theVEGF-460T/C and+405G/C SNPs were analyzed by PCR-RFLP andPIRA-PCR. Statistical analysis was performed using SPSS13.0softwarepackage. A probability level of5%(P<0.05) was considered significant.Results:1The genotype frequencies of the VEGF-460T/C and VEGF+405G/C in conerol belong to Hardy-Weinberg balance,The frequency ofsmokers in lung cancer patients and healthy controls were59.6%/35.4%,(χ~2=6.463, P＜0.01). smoking may increased the risk of developing lungcancer(OR=2.966,95%CI=1.208-70282)Control of patients with lungcancer patients and healthy group, have a family history of the individualratio of39.7%,28.5%(χ~2=6.734, P <0.01). Therefore, prompt a familyhistory of a significant increase in risk of lung cancer.2The genotype frequencies of the VEGF-460the T and C allelefrequencies were56.7%/59.8%and41.3%/40.2%. Respectively. Therewas no statistically significant difference in the genotype distributions orallele frequencies of VEGF-460T/C between the patients and controls(P=0.238). T/T, C/T and C/C in patients and controls were39.6%/34.6%,36.9%/50.4%and23.5%/15%, respectively; Compared with the T/T+C/Tgenotypes, the C/C genotype could no significantly modify the risk of lungcancer. The odds ratio was0.807(95%CI=0.655~1.153).3The genotype frequencies of the VEGF+405respectively; the Gand C allele frequencies were73.8%/43.2%and46.2%/57.6%,respectively. There was statistically significant difference in the genotype distributions or allele frequencies of VEGF-460T/C between the patientsand controls (P=0.001). G/C, G/C and C/C in patients and controlswere31.9%/19.2%,43.8%/46.3%and24.3%/34.6%, Compared with theC/C+C/A genotypes, the A/A genotype could significantly modify the riskof developing epithelial ovarian cancer. The odds ratio was2.005(95%CI=1.339~3.000).4VEGF405G/460C haplotypes significantly increased lung cancerrisk, the OR value of1.32(95%CI=1.08to1.96).Conclusions:1Smoking and Family history may increase the risk of developinglung cancer.2VEGF+405G/C was associated with an increased risk of lungcancer.But VEGF-460T/C none.3VEGF405G/406C haplotypes may increase the risk of developinglung cancer.