The Study On Immunologic Effective Of Elcctrospun PLGA Anti-adhesive Film

Peritoneal adhesions develop after nearly every abdominal surgery, which place atremendous burden on public health. There are massive number of abdominal adhesionprevention strategies developed over the past several decades, however, use seprafilmis an easy and effective method. The electrospun PLGA anti-adhesive film that used inthis study is a biodegradable polymer, it will be decomposed into CO2and H2O in vivo.Nontoxic and nonirritant PLGA has excellent biodegradability, biocompatibilityand mechanical properties. Therefore, the PLGA anti-adhesive film were regard as theanti-adhesive biomaterial which has extensive development space.Biomaterials are playing more and more important roles in the aspect ofsubstituting for tissue or organ, the biocompatibility is the key factor for thebiomaterials' study. With the application of the biomaterials,its evaluation lever isincreased from "acceptable" to "safe to use",so a great deal of studies is being focuson how to set up a set of high efficiency,sensitive,exact and secure evaluation system.The researchers gradually know that the reciprocity between immune system andbiomaterials is important, and the inflammation is also an emphasis,which can reflectthe reaction of the body and biomaterials. In this study we selected the cell viability,cytokines,chemokines as the object,and studies immunological evaluation on theinflammation induced by biomaterials at the cell and protein level.Main conclusions:(1) The electrospun PLGA anti-adhesive film has lower mechanical strengththan commercialization anti-adhesive film, however, tenacity of which is higher thanthe latter. And all mechanical property of the electrospun PLGA anti-adhesive film aresimilar to organism tissues.(2) Due to the special hydrophobicity of the electrospun PLGA anti-adhesive film,it's not conducive to cell adhesion. It is a good foundation for prevent postoperativeperitoneal adhesions formation. The membrane can be biodegradable in vivo, so thedegradated products can directly be englobed by peritoneal macrophage. (3) High concentrations of degradation solution(0.32mg/mL) treatment onmacrophage can reduce cell viability, but the extent of the damage in cells within thetolerance range, macrophages can be adjusted by starting the self repair mechanism.Degradation solution will stimulate macrophage inflammatory cytokines secretionincreased significantly such as IL-1beta, IL-6and TNF alpha, which shows that thematerial has a immunogenicity. Furthermore, in the experiment, the concentration ofdegradation solution is much higher than the membrane application in vivo, so it canbe concluded that the material used for the prevention of postoperative peritonealadhesions does not cause harmful inflammatory response.(4) The results of animal experimental hematology testing, peritoneal lavage fluidcytokines testing and detection of serum cytokines are consistent. Both the electrospunPLGA anti-adhesive film and commercial anti-adhesive film have good anti-stickingeffect, however from the immunological and histological point of view, adhesionprevention effect of electrospun PLGA anti-adhesive film is better than the commercialfilm, and without causing a severe immune rejection.