Effect And Mechanism Of Quercetin On Invasion And Metastasis Of Highly Metaststic Hepatocarcinoma Cell

Objective:Effects and mechanism of quercetin on proliferation, apoptosis, Invasion and metastasis of hepatocarcinoma cell line HCCLM6were studied so as to illustrate the anti-tumor mechanism of quercetin, and further theoretical evidence of which as an anti-tumor drug may be provided.Methods:1.HCCLM6cells dealt with different concentrations quercetin (20,40,60,80,100μmol/L) were then analyzed by MTT assay to calculate the cells'survival rate.2.Apoptosis rate of HCCLM6cells induced by quercetin were detected by flowcytometry.3.The change of HCCLM6cells'adhesion force was analyzed by tumor adhesion test.4.After treatment with quercetin, expression of E-cadherin in HCCLM6cells were tested by immunofluorescence.5.The change of HCCLM6cells's motility and invasion ability were analyzed by Transwell.6.Statistics Methods:Statistic analysis was performed with SPSS12.0. All data in this experiment were indicated Mean±STD.Results:l.Quercetein inhibite the proliferation of HCCLM6cells.By MTT analysis, it is shown that proliferation of HCCLM6cells was significantly inhibited by quercetein at concentrations of40,60,80,100μmol/L (P<0.05), and showing a dose-and time-dependent manner.2.Quercetin can induce HCCLM6cell apoptosis.After treatment with40,60,80μmol/L quercetin for48h, apoptosis rate of HCCLM6cell showed a dose-dependent manner. After incubated with40μmol/L quercetin for48h, the apoptosis rate of HCCLM6cells increased from2.03%of control group to47.44%(P<0.01).3.Quercetin can reduce HCCLM6cells's motility and invasion.After treatment of quercetion, the number of HCCLM6cells that through the artificial basement membrances had a significantly reduction. The inhibitory rate of HCCLM6cells arrived to49.05%(P<0.001). By MTT analysis, it is shown that the adhesion ability of HCCLM6cells was significantly inhibited by quercetein, the inhibitory rate of HCCLM6cells increased from100%of control group to41.88%(P <0.001). After treatment with quercetin, the protein expression of E-cadherin in HCCLM6cells showed a bemarkedlyincreased by immunofluorescence.Conclusions: Quercetin can inhibit proliferation, induce apoptosis, and reduce hepatocarcinoma cells adhesion, invasion and motility ability. The quercetin resistance mechanism of invasion and metastasis in tumor may be related to the increase of the protein E-cadherin. Quercetin might be a potential of liver cancer drugs.