The Research Of Effects Of Combination Of1251Radioactive Particles With Cisplatin Or BLM On Tca8113and ACC Cells In

Objective:Investigate the joint application of125I radioactive particles with cisplatin and PYM on cultured human tongue carcinoma Tca8113cells and adenoid cystic carcinoma cell proliferation.Provide a theoretical basis for clinical125I radioactive seed brachytherapy treatment and the combination of chemotherapy drugs.Methods:(1) Conventional cultured human Tca8113cells and ACC cells.(2) Experimental groups:Figures1,2on behalf of the Tca8113cells and ACC cells; Capital letters represent the different approach, A is not dealt with, B is2.5mg/1cisplatin treatment72h, C is lmg/1Pingyangmycin handle72h, D is the radioactive125I seeds72h, E is the radioactive125I seeds combined2.5mg/1cisplatin treatment72h, F is the radioactive125I seeds and1mg/l Pingyangmycin handle72h.(3) Using an inverted microscope to observe the morphological changes of cells in each group.(4) Using transmission electron microscopy to observe the ultrastructural changes of cells in each group.(5) Detect the rate of apoptosis of cells in each group by flow cytometry (FCM)(6) By immunocytochemical staining to detect the expression of cell protein factor Bcl-2and Bax in each group.(7) Using SPSS11.5statistical software for statistical analysis.Results:(1) All groups of cells were observed under inverted microscope handle72h morphological changes:Cisplatin, BLM, and/or125I radioactive particlesTca8113cells treated rounded, shrinkage, nuclear agglutination and the cytoplasm condensed into a bubble of varying sizes in intracellular vacuole-like structures, most of the cell membrane is still intact, and the number of cells compared with control group significantly reduced celldetached from the culture bottle sidewall, suspension cells increased the number of apoptotic cells.Fl, F2group B, and C and Group D, the number of apoptotic cells increased significantly. Cisplatin, BLM, and/or125I radioactive seed processing after the ACC cell rounded, shrunken, nucleoli disappear into irregular-shaped spindle, the cell body increases, the number of apoptotic cells, compared with the rest of the the number of apoptotic cells was significantly increased.(2) Rate of apoptosis in the TUNEL method of flow cytometry to detect the expression handle72h:Group Al, A2was4.8%,2.4%;B1, B2was10.9%,12.9%;C1, C2was15.7%,17.2%;D1, D2was14.5%,25.3%; E1, E2was36.4%,44.3%; Fl, F2group was53.7%,59.4%.The results showed that after cisplatin, BLM, and/or125I radioactive seed processing, Tca8113cells and ACC cells apoptosis rate increased, drug release of the combined group and drug group, radiotherapy group compared with the apoptosis rate increased the most obvious, with statistical significance.(3) Immunocytochemical staining of cells in each group to deal with72h Bax and Bc1-2expression results:Tca8113cells and ACC cells expression of Bax after cisplatin, BLM or/and125I radioactive particles processing enhanced expression of Bcl-2expression was decreased, and the drug release of the combined group and drug group, radiotherapy group compared, Bax expression increased and Bcl-2expression decreased the magnitude of the most significant.Conclusion:125I radioactive particles in combination with cisplatin or Pingyangmycin human tonguesquamous cell carcinoma in vitro Tca8113cells and salivary adenoid cystic carcinoma of the growth inhibition is more effective than single chemicals and radiation effects;adenoid cystic carcinoma cells than the Tca8113tumor cells more sensitive to125I radioactive seed brachytherapy.