Notoginsenoside-Rg1for Lipopolysaccharide Induced PC12Cells Damage The Research Of Protection

Objective By constructing lipopolysaccharide induced PC12cells of the inflammation damage model, from the cells, the molecular level is to discusse notoginsenoside Rgl for PC12cells inflammatory lesions of protection.Methods Will the in vitro culture pc12cells into the control group, the lipopolysaccharide experimental group, notoginsenoside Rgl and lipopolysaccharide experimental group.The use2',7'-dichloro-fluorescin diacetate Probe in fluorescence spectrophotometer test cells reactive oxygen species level; The Hoechst33342/PI nuclear fluorescent dye technology in fluorescence microscope double under observed count apoptosis and/or necrosis of cells.Results Lipopolysaccharide can induction PC12cells inflammatory lesions, leading to PC12cells active oxygen levels rise, the apoptotic cells and/or necrosis cell number increase, different doses notoginsenoside Rgl can restrain the cell active oxygen rise, the apoptotic cells and/or necrosis cell number increased.Conclusions Lipopolysaccharide can cause the ideal cells inflammation damage model, notoginsenoside Rgl have the protective effect for lipopolysaccharide induced PC12cells inflammation damage.