Establishment Of Piglet Model With Acute Lung Injury Induced By Oleic Acid

Objective:To develope a stable and reproducible piglets model of acute lung injury induced by small injections of oleic acid.Methods:The conventional method of establishing oleic acid-induced acute lung injury model was modified:Oleic acid(Cat NO01008; Sigma St Louis, MO,Purity:99.96%)0.1ml/kg was melted in the same volume of absolute alcohol, firstly mixed with10ml self-made piglet serum. Then the diluted oleic acid was infused into the pulmonary circulation within15minutes slowly and steadily,using a syring connected to the jugular venous cannula.12piglets were randomized into two groups:Study group (Oleic acid was administered with the above method) and Control group (Saline was administered as control). And both groups hemodynamic were stable for at least4h. Hemodynamic and pulmonary function data were monitored. Histopathology of lung tissue was detected. Results:There is no significant changes in hemodynamics and pulmonary function in Control group. Study group showed the moderate decrease in blood pressure and cardiac output. Study group showed sever lung injury in pathologic.Conclusion:With the use of modified injection of oleic acid, a stable model of early respiratory distress in piglets can be achieved. Such a stable model allows for a diversity studies on early respiratory distress.