The Relative Research Of DKK-1in Active Rheumatoid Arthritis

Objectives: It aims to detect the dickkopf-1(DKK-1) levels in serum of patients with activeRheumatoid arthritis (RA), to probe into the role of DKK-1which plays in active RA, byanalyzing relationship between DKK-1and the clinical symptoms of RA, as well as experimentindexes, and to provide a reference basis for monitoring the RA progress, and a new clinicalindex which can reflect the bone erosion degree.Methods: There are76cases selected as subjects, including35cases of patients with RA (inactive period of illness),21cases of patients with non-RA rheumatism, and20cases of healthycontrols. It takes the microporous board, covered by purified DKK-1monoclonal antibody as asolid phase antibody, checks the DKK-1level in blood serum with ELLSA method, and probesinto the role of DKK-1in RA, by analyzing the relations between DKK-1and clinical symptomsof RA, as well as doing relative analysis with lab indexes.Results:(1) The DKK-1level (4.67±2.02ng/ml)in RA group is apparently higher than that ofnon-RA rheumatism group (3.62±1.57ng/ml)and healthy controls (1.91±1.86ng/ml), which isof statistical significance (P<0.01).(2)The DKK-1level of RA group is related to anti-CCP ESR CRP DAS scores, andrelevant coefficients are respectively0.622,0.580,0.378, and0.646(P <0.05); on the other handit has no relation to PLT, age, duration, and relevant coefficients are respectively0.158,0.239,0.083(P>0.05).(3) In three groups divided by RF drops, DKK-1level was not statistically different. ANAnegative group has higher DKK-1level than ANA positive group, which is of statisticalsignificance(P <0.05).Conclusions: DKK-1level is related to RA patients' inflammation and RA bone erosion. Thechange of DKK-1level can be used as an index of RA patients' diseases and bone erosion.