Influence Of Irbesartan On The Expression Of Monocyte Chemoattractant Protein-1 In The Kidney Of Diabetic Rats

Objective:To observe the effects of irbesartan on the expression of monocyte chemoattractant protein-1 in the kidney of diabetic rats and assess its renal protective effect.Methods:The rat model of type 2 diabetes was established by intraperitoneal injection of STZ (30mg/kg) and feeding with high-glucose, high-fat diet. Thirty Wistar rats were divided randomly into normal control group (A group), diabetic group (B group) and Irbesartan treated group (C group). At the end of the 12th and 16th weeks, samples were collected, including blood glucose (BG), cholesterol (CH), triglyceride (TG), blood urea nitrogen (BUN), serum creatinine(Scr) and 24-hour urinary protein excretion(24Upro). All rats were sacrificed at the end of the 16th week in each group. kidney/body weight ratio (KW/BW) was measured.The structure of the kidney was examined by light microscopy. The expressions of MCP-1mRNA and protein in the kidney were detected by Real-time Fluorescent Quantitative PCR and immunohistochemical methods.Results:(1) Compared with the normal control group. BG, CH, TG, BUN, Scr,24Upro, KW/BW and the expressions of MCP-1mRNA and protein increased significantly in the diabetic group (P<0.01). (2) In the group treated with Irbesartan, BG, CH, TG, BUN, Scr,24Upro, KW/BW and the expressions of MCP-1mRNA and protein decreased significantly compared with the diabetic group at the end of the 16th week (P<0.05, P<0.01).Conclusions:Irbesartan can effectively delay the progression of the diabetic kidney disease by reducing the expression of MCP-1mRNA in the kidney of diabetic rats.