The Slc2a9 Gene Eighth Exon 137a / G Polymorphism And Diabetic Patients With Hyperuricemia Relationship

Objective:To investigate the single-nucleotide polymorphism(SNP) at position 137A/G of exon 8 of SLC2A9 gene for the association with diabetes mellitus and hyperuricemia in the Chinese Han population in Qingdao City of Shandong province.Methods:A segment of exon 8 of SLC2A9 gene was amplified by sequence specific primers and polymerase chain reaction (PCR) method, then all PCR products were digested by restriction enzyme BstUI and a part of PCR products were submitted to DNA sequence analysis, and we detected the differences of the allele frequency and genotype frequency of SLC2A9 gene at position 137A/G in 414 patients in affiliated hospital of medical college of Qingdao university in Shandong province.Results:A allele frequency of 137A/G polymorphism of exon 8 of SLC2A9 gene in control group is 68.1%, in hyperuricemia group is 58.6%, in diabetes mellitus group is 63.6%, and in diabetes mellitus and hyperuricemia group is 58.2%, while G allele frequency in control group is 31.9%, in hyperuricemia group is 41.4%, in diabetes mellitus group is 36.4%, and in diabetes mellitus and hyperuricemia group is 41.8%. The difference of allele frequency between control group and hyperuricemia group had statistical significance(P<0.05), the difference of allele frequency between control group and diabetes mellitus and hyperuricemia group had statistical significance (P<0.05), the difference of allele frequency in other groups had no statistical significance (P>0.05).The AA genotype frequency in control group is 45.1%, in hyperuricemia group is 36.2%, in diabetes mellitus group is 41.7%, and in diabetes mellitus and hyperuricemia group is 30.8%, the AG genotype frequency in control group is 46.1%, in hyperuricemia group is 44.8%, in diabetes mellitus group is 43.7%, and in diabetes mellitus and hyperuricemia group is 54.8%, and the GG genotype frequency in control group is 8.8%, in hyperuricemia group is 19.0%, in diabetes mellitus group is 14.6%, and in diabetes mellitus and hyperuricemia group is 14.4%. The genotype frequency in those groups had no statistical significance (P>0.05).Conclusion:In the Chinese Han population in Qingdao City of Shandong province,137A/G polymorphism of exon 8 of SLC2A9 gene is assicated with diabetes mellitus and hyperuricemia, the incidence of diabetes mellitus and hyperuricemia in those people with A allele is higher than those with G allele.