Qingdao, Shandong Cervical Lesions Hpv16 Urr And E6 Gene Mutation Analysis

ObjectiveThe study was performed to investigate genetic variants of the HPV16 URR and E6 genes derived from Qingdao women with different stages of cervical lesions, and to assess the association between the sequence variant and the cervical lesions.Materials and Methods[1] We use the technology of polymerase chain reaction (PCR) to detect 120 cases of cervical cancer,254 cases with cervical intraepithelial neoplasia (CIN). Then we selected samples with HPV16 positive, and amplifies the HPV16 URR and E6 genes. The products of PCR were purified and sequenced, and then were compared with the standard strain.[2] DNA samples were extracted from the cervical cancer tissues.[3] The extracted DNA samples were amplified by polymerase chain reaction(PCR) using specific primers for high-risk HPV(MY09/11), HPV16 to identify the positive tissues.[4] DNA extracted from the HPV16 positive tissues were amplified by the primers for URR and E6, then the PCR fragments of HPV16 URR and E6 genes were purified, sequenced and compared with the standord stain.Results[1] HPV16 was present in 60.00%(72/120) of cervical cancers,43.10%(50/116) in CINIII and 23.19%(32/138) in CINII. The distribution of HPV16 in Qingdao were signi?cantly associated with cervical neoplasia development (P=0.000).[2] There was 19 kinds of mutations in the URR gene. The mutation at nt 7521 from G to A was most frequent. A remarkable finding was the co-nutations including nt 24, nt 7730 and nt 7842. The most frequent sequence variation of E6 was nt 178, which was 61.11%(44/72),62.00%(31/50)å'Œ50.00%(16/32) in Cervical canser, CINIII and CINII. The mutation of L83V was only found in 6 cases. In Qingdao area, two major branches of HPV16 were type AS 59.09%, followed by proptype 33.12%.Conclusions[1] HPV16 is associated with the progression of cervical cancer.[2] The mutational hot spots of HPV16 URR are nt7521 and a co-mutation including nt24, nt The mutational hot spots of HPV16 URR are nt7521 and a co-mutation including nt24, nt7830 and nt7842. The hot spot of E6 was nt178.[3] HPV16AS lineage is predominant in Qingdao. As lineage appeares to be more oncogenic than E lineage.