| Background:The bone marrow mesenchymal stem cells (Mesenchymal stem cells, Mscs) have multiple differentiation potentials.They could be divided into many different organizations with different induction. Mscs have become the most common seed cells in the process of tissue-engineered cartilage construction in recent years.Objective:To Investigate the role of the intra-articular environment to the Mscs by studying the influence of exogenous hyaluronic acid (hyaluronic acid, HA) on proliferation and chondrogenic differentiation of rabbit bone marrow mesenchymal stem cells.Methods:The rabbit Mscs were isolated and cultured in the method of whole bone marrow and adherent culture. The 4th passage cells were used for the experiment. The experimental group cells were induced by HA solution, with TGF-β3 induced group as a positive control. The negative control group was joined the regular medium. The features of chondrocytes were identified by toluidine blue staining,immunohistochemistry and RT-PCR to detect the expression of collagenⅡafter 7,14,21d's induction respectively.Results and Conclusion::After induced by HA, the speed of cell proliferation slowed down. The cell morphology changed from long spindle to polygonal, oval. The extracellular matrix showed metachromasia with toluidine blue and positive with typeⅡcollagen immunohistochemical staining. RT-PCR detection also showed the expression of typeⅡcollagen mRNA. All the above showed the characteristics of cartilage cell differentiation. But their expression were weaker than the positive control group. The results indicated that the exogenous HA can induce the rabbit Mscs differentiate to chondrocytes. But its capacity is weaker than TGF-β3. Therefore, the intra-articular environment played a positive role in chondrogenic differentiation of Mscs and supported that HA could be used as a matrix for cartilage tissue engineering. |
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