| Antler Base has a high medicinal value. Over the past years people used it to treat mastitis, mumps and boils. Clinical applications have confirmed that Antler Base has sound anti-inflammatory effect. But there is no report for its anti-inflammatory mechanism. So this paper proposed to study the anti-inflammatory effect and its mechanism of Antler Base Extract by animal models and cell experiments.1. Xylene-induced ear edema mice model was used to evaluate the anti-inflammatory effect of Antler Base powder suspension(2.57 g/kg/d), ethanol extract of Antler Base(0.12 g/kg/d) and aqueous extract of Antler Base(0.44 g/kg/d), hydrocortisone(6 mg/kg/d) as a positive control. The results showed that Antler Base powder suspension group had lower swelling rate(132.46±15.26%) than the control(170.19±24.68%)(P<0.05). Aqueous extract group and hydrocortisone group had lower swelling rate (65.43±11.95%&64.63±11.73%) than the control (P<0.01). But ethanol extract didn't have this effect.2. Carrageenan-induced paw edema mice model is one of the most classical models of anti-inflammatory drug research. This model was used to study the anti-inflammatory effect of aqueous extract of Antler Base. The results showed that high-doses aqueous extract of Antler Base group (0.44 g/kg/d) and hydrocortisone group (6 mg/kg/d) had lower swelling rate (43.68±2.32%&40.50±2.45%) than the control 52.91±3.66%(P＜0.01). Low-doses aqueous extract of Antler Base group (0.18 g/kg/d) had lower swelling rate (48.46±2.22%) than the control (P＜0.05).Aqueous extract of Antler Base could also reduce the content of serum NO and MDA, reduce the content of PGE2 in leachate of inflammation foot and enhanc the activity of LZM, SOD and GSH-PX in serum. Its anti-inflammatory effects is probably by inhibiting the over-expression of NO to reduce cell trauma, enhancing LZM activity to improve the metabolism of polysaccharides, enhancing SOD and GSH-PX activity to reduce lipid peroxidation to reduce tissue damage and promote inflammatory tissue injury and recuperate. Aqueous extract of Antler Base could significantly reduce the content of IL-6 in leachate of inflammation foot, but it didn't affect IFN-y and IL-2 levels significantly. The results showed that aqueous extract of Antler Base might be playing anti-inflammatory effects by inhibiting monocyte-macrophage activation.3. Cultured·the LPS-induced RAW264.7 cells at 0-40 mg/mL concentration of aqueous extract of Antler Base for 12h, then analysis the content of NO,PGE2 and IL-6 in cell culture medium. The results showed that as the concentration of aqueous extract of Antler Base increase, the content of NO, PGE2 and IL-6 in cell culture medium decreased in a dose-dependent manner.The results show that aqueous extract of Antler Base has significant anti-inflammation effect. This effect may occur by inhabiting the body's over-expression of inflammatory mediators, inhabiting of macrophage-related cytokines and enhancing the activity of the related enzymes of body. |
