| Objective:To explore the anti-embryo implantation effect of ginkgolide B.Methods:(1)Two kunming female mice without producing, the two sides of uterine horn of which were taken out immediately for immunohistochemical observation after being killed by cervical disconnecting. (2) There were twenty-eight kunming female mice without producing in this experiment.Twenty-five mice were randomly divided into five groups. On the 2nd day of pregnancy, the mice were given ginkgolide B in different doses in one side of the uterine horn (10μl,20μl,30μl,40μl,50μl) as the experimental side, equal amount of physiological saline were injected into the uterine horn of the other side as the control side. On the 8th day of pregnancy, the mice were executed by cervical disconnecting for counting the number of embryos and the two sides of uterine horn were taken out immediately for pathology, immunohistochemical and transmission electron microscopy observation;The other three mice without any treatment were taken as the blank control group.Results:(1)PAF-R exist extensively on the endometrium;(2) Compared with the control side, the groups,with ginkgolide B (20μl,30μl) injected in the uterine horns, had a significantly reduction about the number of embryos (P<0.05), accompanied with the decidua becoming thinner. The expression of immunoreactive positive material(IPM) of laminin (LN) and fibronectin (FN) reduced and the growth of the mouse embryos were retarded, even retrogressivly degenerated; under the electron microscope, we can see that some small vacuoles had appeared, the cytoplasms of trophoblastic cells became less dense and the morpha of the nucleus changed; the lysosome increased greatly within the cytoplasms of decidua cell and the mitochondria had oedema. In the group of large doses of ginkgolide B(≥40μl), the number of embryos had a even greater reduction(P< 0.001).In some cases, the endometriums had no decidual reaction,no embryo planting, and few of expression of IPM of LN and FN; meanwhile the lysosome increased greatly within the cytoplasms of trophoblastic cells, the nuclear became karyopyknosis and fragmentation; the cytoplasms of decidua cells had the same change,and the nuclear membrane were solubilied. (3) In the experimental side,accompanied with the increased doses of Gink-golide B, the number of embryos and the expression of IPM of LN and FN were down tendency (P<0.001). (4)Compared with the blank control group, the control groups had a slightly reduction about the number of embryos,but had no significant differences between them (P>0.05)Conclusion:As a PAF antagonist, ginkgolide B had the effect of anti-embryo implantation and being worthy of further clinical researching and applications. Objective:To explore the anti-early embryo development effects of ginkgolide BMethods:Twenty kunming female mice without producing were randomly divided into group A and group B. Between the 7th to 10th day of pregnancy, ginkgolide B (0.07ml) was intraperitoneally injected to the A group of mice continually (as the experimental group); equal amount of physiological saline was injected intraperitoneally to the B group as the control group.On the 19th day of pregnancy, the mice were executed by cervical disconnecting for counting the number of embryos and the two sides of uterine horn, ovary, heart, liver, kidney were taken out immediately for pathology observation.Results:Compared with the control group, the group injected intraperitoneally ginkgolide B had a significantly reduction about the number of mouse embryos (P< 0.01),and the embryo stoped developing. The decidual of the mouse's utera became edema or being infiltred by inflammatory cell; The ovary.the heart,the liver and the kidney of the mouse had not any pathological change during this experiment.Conclusion:As a PAF antagonist, ginkgolide B had the effect of anti-early embryo development and being worthy of further clinical researching and applications. |
PDF Full Text Request