Expressions Of TβR Ⅰ Infrontal Lobe And Hippocampus Tissues Of Kainic Acid-induced Epileptic Rats

Objective: Epilepsy is a group of common neurological chronic diseases with complicated nosogenesis. Some investigator had proposed a new probalble pathological mechanism for epilepsy. Large numbers of serum proteins penetrate into extracellular space in brain tissues through the injured blood-brain barrier (BBB) with increased permeability. Serum proteins develop an interaction with transforming growth factorβreceptorⅠ(TβRⅠ) which distributes widely in cellular membrane of astrocytes, regulating the function of astrocytes and inducing epilepsy eventually. Whether TβRⅠis involved in the pathogenesis of epilepsy, it needs a lot of experimental data to support. This paper aims to study the expression of TβRⅠin frontal lobe and hippocampus tissues of kainic acid(KA)- induced epileptic rats, in order to probe the relationship of TRβⅠand epilepsy, and then to provide further experimental evidences for whether TβRⅠparticipates in the pathogenesis of temporal lobe epilepsy(TLE).Methods: 60 male SD rats were randomly divided into two groups, model group (50) and control group (10). According to the time when the experimental animals were killed, model group was divided into five subgroups(6h, 12h, 24h, 72h, 1w). Animal models of TLE were established in SD rats by intracerebroventricular injection of KA (1μg/μl), the same volume of saline was injected into the rats of control group. Behavioral observation was performed with Racine Classification Standard and rats of gradeⅢ-Ⅳseizures were accepted into model group. Expressions of TβRⅠmRNA and TβRⅠprotein in frontal lobe and hippocampal tissues within various phases after seizures were measured by Semi-quantitative RT-PCR method and Western Bloting Technology.Results:①Compared with control group, TβRⅠmRNA expression of model group increased significantly in frontal lobe and hippocampus tissues after seizures;②Consisting with results of semi-quantitative RT-PCR, TβRⅠprotein expression of model group were significantly higher than those of control group in frontal lobe and hippocampus tissues after seizures;③Expressions of TβRⅠmRNA of model group started to increase gradually at 6h and reached the peak at 72h and decreased gadually on 1w after seizures. Expressions of TβRⅠmRNA consisted with the expression of TβRⅠprotein in model group;④Expressions of TβRⅠmRNA and TβRⅠprotein in hippocampus tissues was more higher than those in frontal lobe;⑤No significant differences of TβRⅠmRNA and TβRⅠprotein expression were found between left side and right side of frontal lobe and hippocampus.Conclusion: Abundant TβRⅠmRNA and TβRⅠproteins were expressed in frontal lobe and hippocampus of TLE model group, indicating that TβRⅠmay be involved in the mechanism of epilepsy.