Vitc In Urinary Bladder Cancer Cell T-24 Chemotherapy Efficiency And Function Mechanism Research

Objective:To investigate THP combination with VitC impaced on proliferation, apoptosis of Bladder cancer cell (T-24)and expression of Caspase-3mRNA,SurvivnmRNA in Bladder cancer cell (T-24). Thus to provide the theory basis for the clinical application of VitC as bladder perfusion adjuvant chemotherapy drugs in bladder perfusion chemotherapy. Methods:1. To treat bladder cancer cell (T-24)with the different concentrations of VitC combination with the constant concentration of THP in vitro, and to study proliferation of T-24 cell by cell proliferation curve method.2. The chang of the cell cycle and apoptosis of them was analyzed by flow cytometry after T-24 were treated for 48 hours in vitro with VitC combination with THP.3.The expression of the SurvivinmRNA of them was detected by RT-PCR method after T-24 cells were treated with the certain concentrations of VitC combination with the different concentration of THP for 48 hours in vitro.4.The expression of theCaspas-3mRNA of them was all examined by RT-PCR method after T-24 cells were treated with the certain concentrations of VitC combination with the fixed concentration of THP for 48 hours in vitro.Results:1. It was showed that adding THP or VitC(400ug/ml) alone may inhibit significantly proliferation of T-24 cell compared with the control group, and THP combinatiom treatment with VitC inhibit the proliferation of T-24 cell more obviously than THP use alone.2.Tested by flow cytometry,it indicate that Effects of fixed concentration of THP combin with VitC on cell cycle is no regularity,but apoptosis increasedobiviously.3. That RT-PCR detected expression of SurvivinmRNA showed: the expression of SurvivinmRNA decreased obiviously(P<0.01) in T-24 cell treated with THP or alone compared with the control group, while in T-24 cell treated with THP combination with vitc the expression further decreased compare with THP alone. (P< 0.01).4. That RT-PCR detected relative expression of Caspas-3mRNA showed:the expression of Caspas-3mRNA increased obiviously in T-24 cell treated with THP alone compared with the control group, while in the T-24 cell combination with vitc its expression further rose compare with THP alone. (P<0.05). Conclusion:The combination of VitC and THP may strengthenTHP inbition on the proliferation of T-24 cells, superior to THP use alone.Whether the molecular mechanisms is relative to inhibiting Survivin and up-regulating the expression of Survivin Caspas-3 gene to be further confirmed.