NOGO-A And MYF-5 In The Acute And Chronic Spinal Cord Compression Pressure Expression And Sighificance

Objective:Acute and chronic studying rat spinal cord injury at different time points after decompression Nogo-A and Myf-5 expression, this paper discusses the changes Nogo-A, Myf-5 in spinal cord injury after decompression expressive meaning. Methods:Age 80 SD rats, only 200 - male 250g weight not limited. Respectively established the acute spinal cord injury model and chronic spinal cord damage model. Set up A group of normal control group, and group B JiaShouShu group, the group C, D chronic oppression reduced pressure group of acute oppression, the pressure groups respectively after decompression, 3d in 0d 20d 10d, drawn,. Application immunohistochemical method observation oppression spinal cord tissues Nogo-A, atrophy Myf-5 expression gastrocnemius changes. HE dyed observation after spinal cord injury and muscle atrophy morphological changes in different period. Results:Normal control group (group A) and JiaShouShu group (group B) compare Nogo-A expression does not change significantly after decompression, chronic oppression group (group C) and acute oppression group after decompression (D group) Nogo-A and Myf-5 expression changes remarkably, delayed-type greup significance. Immunohistochemical display: Nogo-A group D after rising pressure 0d express 3.34), plus or minus (junior high (91.1 3d expression plus or minus 327), then 10d, 20d expression drop, group C in Nogo-A 0d expression after decompression 95.9 + 4.58 supreme (in), then 20d expression 10d, 3d, dropped to basic level, group D group compared with C cut slowly. Normal control group (group A) and (B) compared JiaShouShu group Myf-5 expression, chronic oppression does not change significantly reduced pressure group (group C), 0d express (17.4 + 1.28) below the normal control group (aerage 22.4 + 1. 2009), 3d express (24.1 + 0.99) above normal control group. Increased 10d 20d expression, highest (48.3 20d expression, plus or minus 1.67), acute oppression decompression group (group D) 0d expression (10.7 + 1.71) group compared with normal no obvious difference, 3d express (16.1 + 1.49) than the expression, then 0d over-active. But D group raised than group C slow. Conclusion:In the spinal cord injury and after decompression, Nogo-A and down-regulate with reduced pressure the extension of time, Nogo-A cut, but still above express normal levels. Explain to the Nogo-A nerve repair and regeneration strong inhibition caused, and spinal cord injury after skeletal muscle atrophy Myf lose nerve that 5 and down-regulate Myf-5 in prevention of skeletal muscle atrophy and muscle cell apoptosis plays an important role in protecting, studies show the same degree of oppression under spinal cord compression pressure, chronic after decompression and acute spinal cord compression and decompression compared after spinal cord compression, chronic after decompression is more advantageous to the nerve and muscle tissue repair.