The Study Of CFTR On Squamous Cell Carcinoma And Adenocarcinoma In Human Lung Cancer

Objective: To study the expression and function of CFTR,AQP5 and MMP-2 in the adenocarcinoma,squamous cell carcinoma of lung during the different differentiation degree.Methods: 70 cases lung cancer specimens were collected in Liaoning Province Cancer Hospital, and the 45 cases specimens of them were diagnosed as adenocarcinoma,squamous cell carcinoma by the clinical pathologist. 27 specimens were squamous cell carcinoma, 18 specimens were adenocar- cinmoma, 30 patients were male and 15 patients were female. The ages were from 44 to 80, and the average age was 60. Radiotherapy and chemotherapy were not done before the operation. HE staining and immunohistochemistry of CFTR, AQP5 and MMP-2 were done for the all specimens. The results of the staining were measured by IPP6 software, and analyzed by SPSS13.0.Results:(1) CFTR expression was significantly distinction in the different degree of differentiation of squamous cell carcinoma and adenocarcinoma. The expression of CFTR was gradually increased in the squamous cell carcinoma along with the lower degree of differentiation, and the expression of CFTR was gradually decreased in the adenocarcinoma along with the lower degree of differentiation.(2) AQP5 expression was significantly distinction in the different degree of differentiation of squamous cell carcinoma and adenocarcinoma. AQP5 expression was gradually increased in the squamous cell carcinoma along with the lower degree of differentiation, and AQP5 expression was gradually reduced in the adenocarcinoma along with the lower degree of differentiation.(3) MMP-2 expression was enhanced in the squamous cell cancer and the adenocarcinoma along with the lower degree of differentiation.Conclusion:(1) The expression of CFTR, MMP-2 and AQP5 were gradually increased in the squamous cell carcinoma along with the lower degree of differentiation. The results suggested that CFTR might be involved in proliferation, differentiation, growth and migration process of them.(2) The expression of AQP5 and CFTR was gradually reduced in the adenocarcinoma along with the lower degree of differentiation. Meanwhile, the expression of MMP-2 was gradually increased in them along with the lower degree of diferentiation, and the results suggested that CFTR might be involved in lung adenocarcinoma proliferation, differentiation and migration .(3) AQP5 expression were significantly higher in Well-differentiated lung adenocarcinoma cells than in poorly differentiated adenocarcinoma cells, and the results suggested that AQP5 might be involved in the secretion of lung adenocarcinoma.