The Relationship Between The Genetic Polymorphism Of Mthfr And Adverse Pregnancy Oucomes

ObjectiveThe aim of this study is to investigate the prevalence of Methylenetetrahydrofolate reductase (MTHFR) gene polymorphism in women with adverse pregnancy outcomes. Meanwhile, we analyze and discuss the associativety and the effect of MTHFR polymorphisms on adverse pregnancy outcomes which may be provide theories to reduce the pregnancy outcome and birth defect incidence, and give some effective precautions of adverse pregnancy humans.MethodsWith a case-control study, MTHFR gene 677,1298,1793 alleles and genotypes were detected by the technique of polymerase chain reaction- restriction fragment length polymorphism (PCR-RFLP) in study group (84 case) and control group (87 case). Then analyze the frequencies of allele and genotype of each group.Results1.Frequencies of C677T genotype and allele we found the frequencies of study group have the CC homozygote were shown lower than control group(P=0.007), but the CT and TT frequencies were increased significantly in study group compared with that of the control group(P <0.05). And the total T allele have prevalence than the C allele (P<0.01),which have a statistical significance.2. Frequencies of A1298C genotype and allele The genotype frequencies of AA,AC,CC in adverse pregnancy outcome humans account for 53.6%,33.3%,13.1%, and in normal case the frequencies are 68.9%,28.7%,2.3%,so there is a significantly difference in both study and controls, and the difference have the statistical significance(P < 0.05).3. Frequencies of G1793A genotype and allele The A mutant allele frequencies in case and control account for 44.6% and 30.5%, which have a higher difference between two groups(P <0.05),but the control case have not achieved the Hardy- weinberg genetic equilibrium.4. Combined MTHFR C677T and A1298C and G1793A we have found most of the mutants were two locus variants both in study group and control group, but three alleles mutant at the same time are not detected.Conclusion1. The results of the study show that the MTHFR 677,1298,1793 polymorphic variation may be become the genetic susceptivitity factor and contribute to the etiology of adverse pregnancy outcomes.2. The allele frequencies in control groups and adverse pregnancy outcomes are different, and the allele were A>C,G>A.3. MTHFR gene combined mutant may be as the genetic factor lead to the high incidence of adverse pregnancy outcome disease.