| Objective1.To detect the expression and correlation of Omi/HtrA2 and COX-2 in the adenomyosis tissue .2.Analysis the relationship of Omi/HtrA2, COX-2 expression and adenomyosis between clinical and biological characteristics.3.To investigate occurrence and development of the apoptosis between the expression of Omi/HtrA2, COX-2 and adenomyosis, in order to provide for a new method and a molecular target,what can diagnosis and treatment the disease better.Materials and methods1. Cases collection:All tissue samples,40 cases in total,which were collected from patients with adenomyosis who receive initial diagnosis and surgical therapy in gynecological department of Jinan Military General Hospital during certain time from January 2010 to October 2010 , were categorized as a study group ,including: adenomyosis lesions (including the ectopic around the muscle membrane lesions) were 26 patients ,aged 31 to 51 years, mean age 41.5±5.6 years.14 cases of proliferative phase, 12 cases of secretory phase.14 cases of adenomyoma, aged 32 to 50 years, mean age 40.0±5.8 years. 8 cases of proliferative phase, 6 cases of secretory phase. And 20 cases of normal muscle tissue in the same period due to surgical treatment of uterine myoma removal as the control group (control group). Aged 33 to 49 years, mean age 44.0±4.5 years. 12 cases of proliferative phase, 8 cases of secretory phase. The age distribution between the groups was no significant difference (P> 0.05). Two groups underwent diagnostic curettage pre-operative, and specimens from the endometrium. All patients were confirmed by pathological examination after surgery.And 12 cases of Uterine muscle tissue at the bottom in the same period due to surgical treatment of Cervical carcinoma in situ as the normal control group. Aged 30 to 50 years, mean age 44.5±4.7 years. 6 cases of the. proliferative and secretory phase endometrium have been confirmed by pathological examination after surgery.All patients enrolled in the group before the first half year had not used steroid or gonadotropin-releasing hormone agonist or other drugs, no other immune and metabolic diseases, no contraindication for IUD expulsion and placement.2. Experimental method:To detected the expression of pro-apoptotic protein Omi/HtrA2, Cyclooxygenase-2(COX-2) in the lesions of adenomyosis,adenomyoma and normal myometrium by flow cytometry.3. Statistical analysis:Establish a database include the mean fluorescence intensity and the results of the clinical determination of biological characteristics that is about Omi/HtrA2, COX-2 by applicating Excel2003.The experimental results using SPSS16.0 statistical analysis package. The experimental results is analyzed using SPSS16.0 statistical analysis package. All results were measured usingΧ±SD, said measurement data by t test between the two groups, a number of groups were compared using single factor analysis of variance, correlation between two indicators of use of Spearman rank correlation, Computing age, pregnancies, normal delivery times, flow times, dysmenorrhea, menstrual flow, B-ultrasound range, combined with endometriosis than in the risk factors (OR) and 95% confidence interval (CI) by non-conditional Logistic regression model that expressed the strength of the association between Omi/HtrA2 , COX-2 and adenomyosis. Significance level was a = 0.05, and with P <0.05 was considered statistically significant.Results1.The Omi/HtrA2 was expressed in the cytoplasm of endometrial epithelial cells. It's showed atrend of decreasing expression of the adenomyosis, adenomyoma (lesions, eutopic endometrium) control group, normal control group; especially adenomyosis (lesions, eutopic endometrium) expression of the most significant, (P <0.05) differences were statistically significant.2.The expression of Omi/HtrA2 proliferation and secretory phase in adenomyosis lesions and the endometrium,both higher than control group, normal control group,and the proliferative phase expression higher than secretory phase, (P <0.05) differences were statistically significant.3.Analysis the expression and the combination of clinical biological characteristics of Omi/HtrA2 in adenomyosis, the expression of Omi/HtrA2 in adenomyosis were significant correlation with patients age, normal delivery times, flow times, dysmenorrhea, (P <0.05)and the differences were statistically significant.4.The COX-2 was expressed in the cytoplasm of endometrial epithelial cells. It's showed a trend of decreasing expression of the adenomyosis, adenomyoma lesions, control group,research group expression Higher than the control group, normal control group,especially adenomyosis lesions expression of the most significant, (P<0.05) differences were statistically significant.But it was not statistically significant in the eutopic endometrium (P> 0.05).5.The expression of COX-2 proliferation and secretory phase in adenomyosis and adenomyoma lesions both higher than eutopic endometrium,and the proliferative phase expression higher than secretory phase, (P<0.05) differences were statistically significant.6.Analysis the expression and the combination of clinical biological characteristics of COX-2 in adenomyosis, the expression of COX-2 in adenomyosis were Significant correlation with pregnancies, normal delivery times, flow times, dysmenorrhea, menstrual flow (P <0.05) and the differences were statistically significant.7.The expression of Omi/HtrA2, COX-2in adenomyosis,adenomyoma were positive correlation, r values were 0.362,0.254 (P<0.05) ,the differences were statistically significant. Conclusion1.The expression of Omi/HtrA2 in adenomyosis,adenomyoma lesions and eutopic endometrium group were significantly higher than normal myometrium.It suggests that it may induce epithelial cell hyperplasia, there by contributing to the occurrence of adenomyosis.2.The expression of COX-2 in the adenomyosis, adenomyoma lesions were significantly higher than normal myometrium;overexpression of tissue in adenomyosis indicated that the double biology effects between endothelial cell proliferation and inhibits apoptosis may promote the formation of lesions of adenomyosis.3.The Omi/HtrA2, COX-2 of expression correlation with menstrual cycle in the adenomyosis,adenomyoma and proliferative phase expression higher than secretory phase, indicated that ectopic endometrium had increased proliferation, suggested that Inhibition of apoptosis, ultimately proliferated and formated the adenomyosis.4.The expression of Omi/HtrA2,COX-2 were related with patient's age, pregnancies, normal delivery times, flow frequency, dysmenorrhea, menstrual flow. Indicated that Omi/HtrA2, COX-2 over-expression may be the potential impact of the p-atient's risk factors.5.The expression of Omi/HtrA2, COX-2 in adenomyosis tissue were positive correlation,It suggest that the pathogenesis of adenomyosis is complicated. Both participate and promote the formation of adenomyosis. |
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