Expression And Significance Of TREM-1 Of Many Organs In Mice With Hyperlipidaemia Severe Acute Pancreatitis

Purpose Through blood samples were taken from rats with hyperlipidaemia severe acute pancreatitis(HAP) and Severe acute pancreatitis(SAP),to observe the dynamic change of interleu-kin-6(IL-6),endotoxin-1,interleu-kin-10 ( IL-10 ),tumor necrosis factor alpha(TNF-α);Through the expression of triggering receptor expressed on myeloid cells-1(TREM-1)in pancreas,lung and jejunum of rats with hyperlipidaemia severe acute pancreatitis(HAP) and Severe acute pancreatitis(SAP) ,to approach the effection of TREM-1 in HAP pathogenesy, and provid a new molecular for HAP.Method 60 healthy male Wistar rats,weighing 340-410g.were randomly divided into four groups:control group(A group),n=20; Severe acute pancreatitis (B group),n=20;hyperlipidemia group(C group.n=20), hyperlipidaemia severe acute pancreatitis(D,n=20).any of the four group were randomly divided into three groups (6h group,12h group,24h group) ; A group and B group:balance feed for 8 weeks;C group and D group:high fat feed for 8 weeks.8 weeks later,A group and C group were operated after flipping pancreas ;B group and D group SAP modle was induced by injection of 5%sterile sodium taurocholate into biliopancreatic duct.Rats Blood sample,pancreatic tissues , lung tissues and jejunum tissues were collected . Rats Blood sample were collected through inferior caval vein after operated to examine TG,IL-6,IL-10,ET and TNF-α;We used AEROSET automatic biochemical analyzer to inspection triglyceride. the amount and the color of ascites; bserving the distend of gaster and the calcifing of intestine; bserving the pancreatic tissues, Schmidt scoring method was used to evaluate pancreatic pathology; The expression of TREM-1 mRNA in pancreatic tissues , lung tissues and jejunum tissues was inspected by RT-PCR test. Result 1.TG :C group and D group were significantly higher than A group and B group in three periods(6h,12h,24h) and had significant changes(P<0.05);B group and A group were no significant changes in three periods(6h,12h,24h)(P>0.05); C group and D group were no significant changes in three periods(6h,12h,24h)(P>0.05);there were no significant changes in three periods(6h,12h,24h)(P>0.05)of each group. 2.AMY :A group and C group were significantly lower than B group and D group in three periods(6h,12h,24h) , A group and C group had no significant changes(P>0.05);D group were significantly higher than B group and had significant changes(P<0.01).3.IL-6,IL-10: B group and D group were significantly higher than A group and C group in three periods(6h,12h,24h) and had significant changes(P<0.05); A group and C group were no significant changes in three periods(6h,12h,24h)(P>0.05); D group were significantly higher than B group and had significant changes (P<0.01).4.TNF-αand ET: B group and D group were significantly higher than A group and C group in three periods(6h,12h,24h) and had significant changes(P<0.05); A group and C group were no significant changes in three periods(6h,12h,24h)(P>0.05); D group were significantly higher than B group and had significant changes ( P<0.01 ) .5.pancreatic pathology scored: D group were significantly higher than the other three groups .6.TREM-1:in pancreatic tissues , lung tissues and jejunum tissues, B group and D group were significantly higher than A group and C group in three periods(6h,12h,24h) and had significant changes(P<0.05); A group and C group were no significant changes in three periods(6h,12h,24h)(P>0.05); D group were significantly higher than B group and had significant changes(P<0.01).Conclusion: 1.AMY was higher and the pancreatic pathology was severity in hyperlipemia then no- hyperlipemia.2.When pancreatitis ,hyperlipemia could encourage the expression of IL-6,IL-10, TNF-αand ET,aggravate pancreas damage and SIRS.3.When pancreatitis ,hyperlipemia could encourage the expression of TREM-1 ,aggravate pancreas damage and SIRS.