Differences Of Real-time Fluorescent RT-PCR And General RT-PCR Assays For Detection Of The Esp Of The Enterococcus Faecalis

Purpose: To compare the sensitivity and specificity by both real- time fluorescent one step reverse transcription-PCR and general two- step RT - PCR assays for detection of the esp of the Enterococcus faecalis.Methods: The real- time fluorescent one step reverse transcription- PCR and general two- step PCR assays were carried out to detect the esp of the Enterococcus faecalis in genes segments. Then sensitivity and specificity o f the two methods were compared.Results: For the esp gene segment, the sensitivity of real- time fluorescent one step RT- PCR was up to 7.5 pg/ul, while the general two- step PCR only 750 pg / ul.Conclusion: The sensitivity of real- time fluorescent one step RT- PCR assay for the esp of the Enterococcus faecalis was 100 times higher than general two- step RT- PC.