| Objective To investigate the Borna disease virus (BDV) infection of viral encephalitis (VE) and schizophrenia (SCH) in Guizhou Province.Methods FQ-nRT-PCR was applied to test P40 fragments of Borna disease virus in peripheral blood mononuclear cells (PBMCs) from 68 patients with VE,38 patients with SCH and 84 healthy subjects. Gene sequences of positive products were tested; the test results were analyzed by software.Results The PBMCs from 68 patients suffering from VE were tested in P40 fragments of BDV.7 cases were positive,including 6 in Zunyi City and 1 in Bijie region. The positive rate was 10.29%(7/68). The PBMCs from 38 patients suffering from SCH were tested in P40 fragments of BDV.3 cases were positive, including 2 in Zunyi City and 1 in Bijie region. The positive rate was 7.89%(3/38).84 healthy subjects were not detected in PBMCs for BDV p40 gene.There was a significant difference in positive rate between viralencephalitis patients and healthy subjects (P=0.009). There was a significant difference in positive rate between schizophrenia patients and healthy subjects(P=0.048). The results of gene sequence and homology analysis were that two kinds of VE and SCH patient's DNA sequence were the same. its homogeneity was 100%. Compared with the strain V, the homogeneity of nucleotides sequences of the patient was 95%, and 5 (nt276 T-C, nt322 T-C, nt348 A-G, nt351 C-T, nt369 C-T) nucleotides occurred silent mutations. Compared with the H1766, the homogeneity of nucleotides sequences of the patient was 96%, and 4 (nt276T-C, nt322 T-C, nt348 A-G, nt351 C-T) nucleotides occurred silent mutations. Compared with the He80/FR, the homogeneity of nucleotides sequences of the patient was 98%, and 2 (nt322 T-C, nt348 A-G) nucleotides occurred silent mutations.Conclusions BDV p40 gene fragments were detected in patients suffering from viral encephalitis in Zunyi City and Bijie region of Guizhou Province. Viral encephalitis were infected by BDV。VE may be interrelated with BDV infection. BDV p40 gene fragment were detected in patients with schizophrenia in Zunyi City and Bijie region of Guizhou Province. Viral encephalitis were infected by BDV。SCH may be interrelated with BDV infection. The BDV P40 gene fragments in patients with viral encephalitis and schizophrenia in Zunyi City and Bijie region of Guizhou Province were the same, which may come from the same source of infection. |
