| Background: Viral myocarditis is a disease mainly of the disease, mainly against CVB-based, and endocarditis may be associated with pericardial disease, myocardial toxins damage the virus may be diffuse or localized inflammation VMC cell infiltration is one of the characteristic pathological changes. VMC with the development of the disease can be divided into acute or chronic, depending on the clinical manifestations and the degree of myocardial injury site. Traditional classified the disease, "Chest, palpitation"and other areas, disease-bit parts in mind that the pathogenesis of multi cult poison by the exogenous hot and humid, the homes in the heart, resulting in systolic blood loss due to damage and to cause chest pain, Chest tightness, palpitations, shortness of breath, fatigue and sickness. In recent years, the disease showed significantly higher incidence trend of a great threat to human health is currently no accurate and effective drug treatment of the disease, although western medicine has made some therapeutic effect, but the process along with western medicine There are some inevitable side effects, the urgent need to find out the accurate and effective non-toxic side effects of prescription medicine and traditional Chinese medicine preparations, To this end our group carefully study a variety of ancient medicine and modern pharmacological studies, compatibility of drug components carefully and conscientiously to carry out a variety of experimental animal studies, careful screening of drugs, in order to ensure drug quality and the scientific validity of animal experiments, The research group from the adriamycin induced myocardial injury in mice experiments confirmed the anti-Ke Saqi B viral capsule is effective for myocardial injury, and it enhanced myocardial injury in mice with their immune system and protect myocardial cells, Reduce myocardial oxygen consumption, reduce serum lipid peroxides, membrane stability, improve the activity of superoxide dismutase, etc. are effective, the experimental data for the experimental planning and carrying out treatment of viral myocarditis in mice provides an accurate Basis, so we long way to go.Objective: To investigate the anti-Coxsackie B viral myocarditis capsule (K-CoxB-JN) Compound polysaccharide (polysaccharide) on adriamycin (ADM) induced myocardial injury in mice. Use of animals randomized control study, while giving different drugs, administered before and after the animals were observed at any time the state, according to the mental state of mice were sacrificed before, the survival rate of mice, were killed after the level of the biochemical indicators and organizational form Changes in other indicators to determine the efficacy and safety of the drug.Methods: 175 male Kunming mice were randomly divided into 7 groups of 25 only. (1) normal group: ip volume of saline; (2) Model group: 1,3 days after the beginning of the experiment ip ADM 1mg/kg; the first 5,7 days ip ADM2mg / kg; the first day of ip ADM 9,11 3mg/kg; s 13,15 days ip ADM 4mg / kg, cumulative dose of 20mg/kg. (3) The polysaccharide of high, medium and low dose group + ADM: the drug were given a dose of 6.4g/kg, 3.2 g / kg, 1.6 g / kg, orally once a day, a total of 15 d. Xinxuekang Group + ADM: given drug dose was 1g/kg, orally once a day, a total of 15d. The original side decoction group + ADM: given drug dose was 9.8ml/kg, orally once a day, a total of 15d. The ip dose of ADM method and model group with the same time. (4) after 24h treatment group withdrawal weight, removal of the eye blood, dissected out the heart weight to calculate cardiac index, simultaneous measurement of CK LDH HBDH AST ALT MDA SOD indicators, the number of daily survival of the mice.Result: The five parameters were significantly higher than the normal enzyme group (P <0.01). Compared with model group, five of each treatment group had significantly lower enzyme, which enzyme polysaccharide significantly reduced the high dose group (P <0.01). MDA, SOD can be seen in the table are lower than model group, treatment group, indicating that drugs have played a certain effect, but the polysaccharide in the dose group and low dose group had no significant differences (p> 0.05), health groups and efforts Decoction of the original parties were no significant differences (p> 0.05), similar to that of its efficacy, but can be seen that the efficacy of high dose of polysaccharide was obvious.Conclusion: 1. Coxsackie B viral myocarditis virus capsule is effective, the experimental results confirmed the enhanced myocardial injury in mice with their immune system, protect the myocardial cells, reducing myocardial oxygen consumption, reduction of serum lipid Quality peroxide, stable biofilm, to improve activity of superoxide dismutase, etc. are effective.2. polysaccharide efficacy of high dose group was evident crime, polysaccharides the efficacy of viral myocarditis is as obvious dose relationship. |
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