Studies On The Chemicalconstituents Of Euonymus Acanthocarpus

Euonymus acanthocarpus Franch., belonged to Euonymus genus, Celastraceae, was selected for phytochemistry and pharmacological investigations, widely distributed in Yunnan, Guizhou, Guangdong, Guangxi and other southern province. The roots of E. acanthocarpus have been used as Chinese folk medicine for rheumatism and strain, leaves have been used for the treatment of lumbar muscle strain, hemoptysis, and trauma.Stems and twigs of E. acanthocarpus were isolated and purified in a combination of chromatographic methods (silica gel column chromatography, Sephadex LH-20 column chromatography, preparative- HPLC, et al.) to afford 60 chemical constituents. Their structures were identified as phenylpropanoids (6), lignans (19), terpenes and sterides (6), flavonoids (7), aryl glycosides (4), aromatic compounds (15) and other compounds (3) by spectroscopic data analysis (ESI-MS, 1H-NMR, 13C-NMR, DEPT, et al.).Among them, EAC-1 was a new compound, 45 compounds were obtained from Celastraceae family for the first time; 3 compounds were isolated from Euonymus genus for the first time; others were isolated from this plant for the first time.Thirty-seven compounds were tested for cytotoxicity against MCG-803 and SK-OV-3 cell lines using CCK-8 assay. EAC-27 showed moderate cytotoxicity against MCG-803 with IC50 value of 21.4μM, other compounds exhibited weak cytotoxicity against these two cell lines.