The Significance Of MicroRNAs Expression In HUVECs Modulated By Endostar

Objective To investigate the migration effects of human umbilical vascular endothelial cells(HUVECs)in vitro interfered by Endostar in different concentrations and the expressions of miR-126,miR-221,miR-222 of HUVECs, and to explore the anti-angiogenesis mechanism of Endostar by microRNAs.Methods The effect on the migration of HUVECs induced by vascular endothelial growth factor (VEGF), which were interfered with different concentrations of Endostar(50,100,200,500ng/ml) , were determined by cell scratch healing assay. The expressions of miR-126,miR-221and miR-222, which were interfered with different concentrations of Endostar(25,50,100,200,500ng/ml) on different time(1/2,1,2,4,8h), were determined by real-time fluorescent quantitation RT-PCR. To identify the effect on microRNAs in HUVECs modulated by Endostar, relative gene expression data were analyzed by 2^-△△CT method.Results①Endostar of different concentrations can inhibit the migration of HUVECs directly and reduce effect of up-regulated migration of HUVECs induced by VEGF. It was in dose dependent manner.②Over expressions of miR-126,miR-221 and miR-222 were detected in the HUVECs.③The expression of miR-126 was down-regulated by Endostar significantly(0.07±0.01～0.56±0.04,P﹤0.01), as compared with that interfered by Endostar before. While the expression of miR-221 and miR-222 were obviously up-regulated by Endostar(1.45±0.06～5.12±0.48,1.42±0.19～6.68±1.51,P﹤0.01), as compared with that interfered by Endostar before.④To the extent of obvervation, the expressions of miR-126,miR-221 and miR-222 was not in dose/time dependent manner by Endostar regulating.Conclusion Endostar can inhibit the migration of HUVECs and can regulate the expressions of miR-126,miR-221 and miR-222 in the HUVECs. Endostar may carry out anti-angiogenesis by its modulating microRNAs.